Lab 6 From Genotype To Phenotype Flashcards
What is a restriction enzyme?
Used to cut parts of the DNA. Used to restrict viral infections
What is DNA ligases?
Used to seal DNA strands together.
Why is transformation important in biotechnology?
Allow the replacement of faulty genes with healthy ones
Why is sterile techniques sometimes necessary?
To minimize the potential for contamination of the experiment
Why did we keep our plasmids on ice?
To keep the plasma membrane closed to calcium chloride
Why did we heat shock our bacteria?
To open the plasma membrane to allow DNA into the cell
Why do linearized plasmid, circular nicked and a supercoiled plasmid appear different sizes when run on the across gel?
Because they travel at different rates through the gel
Linear med slow
Circular Nicked slow
Supercoiled fastest
What is a plasmid?
Circular DNA used to transfer genes into cells
