Lab 4 : Structural Staining Flashcards
1
Q
Acid Fast staining - Cold method procedure:
A
- Begin with heat-fixed smear
- Cover with Kinyoun’s Carbol Fuchsin for 5 min
- Rinse with DH2O
- Decolorize with Acid Alcohol
- Rinse with DH2O
- Counterstain with Methylene Blue for 1 min
- Rinse with DH2O
- Blot dry
2
Q
Acid Fast stain result: RED
A
Acid Fast
3
Q
Acid Fast stain result: BLUE
A
Non-Acid Fast
4
Q
Endospore Staining procedure:
A
- Begin with heat-fixed smear
- Set up water bath
- When water is boiling place a piece of absorbent paper on top of the smear
- Place smear over the water bath and cover it with Malachite Green for at least 7 min. Keep adding Malachite Green and make sure the paper is always moist
- After the 7 min remove smear from water bath and remove the paper
- Rinse with DH2O
- Counterstain with Safranin for 1 min
- Rinse with DH2O
- Blot dry with bibulous paper
5
Q
Which two genera of bacteria produce endospores?
A
Clostridium and Bacillus
6
Q
Endospores
A
Resistant structures made through a process known as sporulation in response to extreme environmental conditions
7
Q
Capsular Staining procedure:
A
DO NOT HEAT FIX
1. Place a drop of Nigrosin on a slide
2. Transfer a loopful of bacteria to the drop of stain and mix
3. Spread mixture with another slide
4. Allow to air dry
5. Cover smear with Safranin for 1 min
6. Rinse with DH2O
7. Blot dry