Lab 4 Flashcards
Digestion
Large molecules broken down to small molecules - absorbed & used by cells - physical & mechanical
What are enzymes?
Biological catalysts - convert specific substrate to products - temperature & pH affect activity
What are transport mechanisms?
Active transport & facilitated diffusion - products enter bloodstream
What is + control?
Proof of positive reaction
What is - control?
Proof of no reaction
Salivary Amylase (Ptyalin)
PRODUCED: salivary glands
SITE OF ACTION: mouth
pH: neutral
REACTION: starch +H2O = moltose
Pancreatic amylase
PRODUCED: Pancreas
SITE OF ACTION: small intestine
pH: basic
REACTION: starch +H2O = maltose
Maltase
PRODUCED: small intestine
SITE OF ACTION: small intestine
pH: basic
REACTION: maltose +H2O = 2 glucose
Pepsin
PRODUCED: gastric glands
SITE OF ACTION: stomach
pH: acidic
REACTION: protein +H2O = peptides
Trypsin
PRODUCED: pancreas
SITE OF ACTION: small intestine
pH: basic
REACTION: protien +H2O = peptides
Peptidases
PRODUCED: small intestine
SITE OF ACTION: small intestine
pH: basic
REACTION: peptides +H2O = amino acids
Lipase
PRODUCED: pancreas
SITE OF ACTION: small intestine
pH: basic
REACTION: fat droplets +H2O = glycerol + fatty acids
Part 1 - tubes contain
1) 1ml water
2) 1ml 0.1% amylase solution
3) 1ml 0.1% amylase solution + 10 drops of HCl
4) 1ml 0.1% amylase solution in boiling water for 5 mins
2ml 0.1% starch- incubate @ 37° for 30mins
Iodine
- amber/brown
+ Blue/black
Benedicts
- blue
+ greenish
++ yellow
+++ brick red
2.5ml added to 2mls solution heated for 10 mins
