Lab 4 Flashcards
Objectives
-Practice making solutions of different concentrations using the formula C1V1=C2V2
-Use your isotonicity observations and basic chemistry knowledge to predict the isotonic concentration of a given solute.
Intro
I will be modeling the transport of permeable and impermeable solutes across biological membranes and then I will compare my model with the movement of water and solutes across the cell membrane of cow red blood cells (RBCs)
3 Factors that are needed for the rate of membrane penetration by a solute
- Molecular size of the solute (permeability decreases with increasing size)
- Lipid solubility (permeability increases with increasing fat or oil solubility)
- Degree of ionization (permeability decreases with increased ionization)
Some solutes that are not soluble but can pass freely because of
selective protein channels or protein carriers
What happens with the osmotic gradient
water will move across the membrane making the relative concentration of impermeable solutes is equal on both sides
Tonicity
The concentration of the non-permeable solutes in the bathing solution relative to the normal concentration within the cell
What happens when there is a solution with greater tonicity?
Solution will have more solutes and less water.
In a solution, as solute concentration increases
water concentration decreases
Hemolysis
The breakage of red blood cells
Operational Definition for the Isotonic Concentration
The lowest concentration of any impermeable solute that produces a cloudy RBC suspension similar to the isotonic standard made in the previous section. This concentration will be either isotonic or a little hypertonic
Range of NaCl solutions
80-135mM
Random shit
0.3ml of diluted red blood cells
3ml of solution
0.5M of sucrose stock solution
C1V1=C2V2
The lowest sucrose concentration that produces a cloudy suspension similar to the isotonic NaCl standard
Is either isotonic or a little hypertonic
