Lab 3.2: Recombinant DNA Technology Flashcards
Is a method used in Genetic Engineering involving the manipulation of DNA to produce a desired trait.
Recombinant DNA Technology
The manipulation of DNA to produce a desired trait.
Genetic Engineering
The first plasmid used by Boyer and Cohen was even named after Stanley Cohen, which is?
pSC101.
The pSC101 serves as a ________ for a genomic DNA fragment during the formation of an rDNA.
vector
Serves as a vector for a genomic DNA fragment during the formation of an rDNA.
pSC101
pSC101’s important feature is a single region with a _______ sequence that can be recognized by an
enzyme to cut it open.
GAATTC
Another important feature of pSC101 is the presence of?
a tetracycline resistance (TC) gene
A feature that is especially important in the isolation of host cells that contain recombinant DNA.
tetracycline resistance (TC)
There are several different types of restriction enzymes, each of which can identify specific nitrogenous base sequences. Give one example.
EcoRI (eco R one)
What gene is neeeded for synthesis of β-galactosidase
LacZ Gene
It cleaves DNA at GAATTCC sequence
Restriction Enzyme
what type of vector is used in DNA recombination?
plasmids
This is a region on the plasmid often example by resistance genes.
Selection Marker
This is a region of a Genome that is inserted in the plasmid.
Gene of Interest
The exposed ends after cleaving at the GAATTCC region.
sticky ends
