Lab 3: Paper Chromatography Flashcards

1
Q

what was the solvent used to see the amino acids?

A

ninhydrin

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2
Q

what was the mobile phase in this experiment?

A

1-butanol, acetic acid, & water

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3
Q

was the mobile phase more or less polar than the stationary phase?

A

less polar

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4
Q

what was the stationary phase used in this experiment?

A

cellulose paper

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5
Q

is cellulose paper polar or nonpolar?

A

polar

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6
Q

how does the solvent move up the paper in chromattpgraphy?

A

through capillary action

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7
Q

list the 9 non-polar amino acids

A
  • glycine
  • alanine
  • valine
  • leucine
  • isoleucine
  • proline
  • phenylalanine
  • tryptophan
  • methionine

FW

M

GAV LIP

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8
Q

list the 11 polar amino acids

A
  • serine
  • threonine
  • histidine
  • lysine
  • arginine
  • glutamic acid
  • aspartic acid
  • asparagine
  • glutamine
  • cysteine
  • tyrosine

NQ

HKR

DE

C

Y

ST

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