Lab 1: Micropipetting Basics Flashcards
1
Q
what is the range of a P2 micropipettor?
A
0.2-2μL
2
Q
what is the range of a P10 micropipettor?
A
1-10μL
3
Q
what is the range of a P20 micropipettor?
A
2-20μL
4
Q
what is the range of a P100 micropipettor?
A
20-100μL
5
Q
what is the range of a P200 micropipettor?
A
50-200μL
6
Q
what is the range of a P1000 miicropipettor?
A
200-1000μL
7
Q
list the steps to properly use a micropipette.
A
- press plunger to 1st stop and hold
- insert into sample & slowly release plunger
- put micropipettor in desired location & slowly press to the 2nd stop and hold
- remove micropipettor from tube then release plunger
8
Q
how many μL are in a liter?
A
10^6
9
Q
how many μL are in a mL
A
1000