Lab 3

Question 1

What does ‘‘pH’’ stand for?

Answer 1

potency of hydrogen

Question 2

What are acids and bases? Know the examples given?

Answer 2

Acids- molecules release hydrogen ion in solution (0-5)
Bases-molecules that can accept free hydogen ion ( form attraction) ( 10-14)
water is weak base and weak acids

Question 3

Know how to calculate the PH of a concentration of an acid or a base, including equations and which ones to use when

Question 4

How is the concentration of H+ related to the pH number ?

Answer 4

The pH scale is a measure of the acidity or basicity of a solution and is defined as the negative logarithm (base 10) of the hydrogen ion concentration.

Question 5

What was the role of Anthocyanin molecules in a lab

Answer 5

Anthocyanin molecules are pigments responsible for the red, purple, or blue colors in many fruits, vegetables, and flower

Question 6

What is a buffer? What do they do?

Answer 6

bind free hydrogen ion

Question 7

What is the normal pH range for human blood?

Answer 7

7.35-7.45

Question 8

Acidosis

Answer 8

Acidosis is a medical condition characterized by an abnormally low pH level in the blood, indicating increased acidity

Question 9

alkalosis

Answer 9

by an abnormally high pH level in the blood, indicating increased alkalinity or a decrease in acidity.

Question 10

Enzyme

Answer 10

An enzyme is a biological molecule, typically a protein, that acts as a catalyst in living organisms.

Question 11

What words describe charged side groups of their amino acids building blocks?

Answer 11

“charged residues” or “charged amino acid side chains.

Question 12

Activation Energy

Answer 12

Activation energy is the minimum amount of energy required for a chemical reaction to occur.

Question 13

Substrate

Answer 13

substrate refers to the specific molecule upon which an enzyme acts.

Question 14

Products

Question 15

Know the difference between a free enzyme and an enzyme-substrate complex

Answer 15

a free enzyme is the unbound, inactive form of the enzyme, while an enzyme-substrate complex is a temporary association formed when the enzyme interacts with its substrate during a chemical reaction. The formation of the enzyme-substrate complex allows the enzyme to catalyze the conversion of the substrate into products.

Question 16

What is the substrate of the alpha-Amylase enzyme?

Question 17

Active site

Answer 17

The active site of an enzyme is a specific region or pocket on the enzyme’s surface where a substrate binds during a chemical reaction.

Question 18

Affinity (low and high)

Answer 18

strength of bond at the active site
high =tight
low=loose

Question 19

substrate specificity

Question 20

Optimal environmental condition

Question 21

How do we use a standard or a standard curve to measure unknowns ?

Question 22

Is aspirin an acids or a base?

Question 23

Define independent and dependent variable? Know how to properly label titles and units to axes of a graph displaying data