Lab 2 (part 2): Staining Techniques

Question 1

_________: used to determine cell morphology, size, and arrangement

Answer 1

simple stain

Question 2

_________: stains that allow microbiologists to distinguish between different groups of bacteria based on their research with the stain

Answer 2

differential stains

Question 3

What are the two types of differential stains?

Answer 3

Gram and Acid-fast

Question 4

________: a stain that determines whether the bacteria has a capsule or slime layer

Answer 4

Capsule stain

Question 5

________: a stain that determines whether the bacteria produces spores

Answer 5

Endospore stain

Question 6

________: a stain that determines whether the bacteria have flagella and are therefore motile

Answer 6

flagella

Question 7

What are the cellular layers of a gram positive cell?

Answer 7

peptidoglycan and cell membrane

Question 8

What are the cell layers of a gram negative cell?

Answer 8

outer membrane, peptidoglycan, and cell membrane

Question 9

Techoic acids will be found on what type of cell?

Answer 9

gram positive

Question 10

LPS will be found on what type of cell?

Answer 10

gram negative

Question 11

porin proteins are found on what type of cell?

Answer 11

gram negative

Question 12

What are the solutions used in gram staining in order of use?

Answer 12

Crystal violet
Iodine
Alcohol
Safranin