Lab 2 Flashcards
1
Q
The specific aim of the two laboratory sessions is to investigate the process of
A
ammonia assimilation into amino acids in the cyanobacterium Synechocystis
2
Q
In Experiment 1 we will investigate the effect of increasing the external supply of nitrogen (from ammonium) on the activity of GS.
A
In Experiment 2 we will investigate the effect of ammonium supply on the concentrations of glutamine, glutamate and 2-oxoglutarate in the cells
3
Q
The first step is catalysed by glutamine synthetase (GS):
A
Glutamate + MgATP + NH3 glutamine + MgADP + Pi
4
Q
The second step is GOGAT
A
Glutamine + 2-oxoglutarate + NADPH 2 x glutamate + NADP+
