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Micro Biology > Lab > Flashcards

Lab Flashcards

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AP® Biology flashcards Biology 101 flashcards
1
Q

Aerobic bacteria(BMN)

A
  1. Bacillus subtilis
  2. Mycobacterium tuberculosis
  3. Norcadia asteroides
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2
Q

Anaerobic bacteria(VELA)

A
  1. Veillonella
  2. Eubacterium
  3. Lactobaccilus
  4. Actinomyces
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3
Q

Facultative anaerobes

A
  1. Staphylococcus aureus
  2. Escherichia Coli
  3. Listeria
  4. Cutibacterium acne
  5. Clostridium botulium
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4
Q

Micraerophiles (C.H.S)

A
  1. Campylobacter jejuni
  2. Heamophilus influenzae
  3. Streptococcus oralis
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5
Q

Aerotolerant (L.S)

A
  1. Lactobacillus rhamnosus
  2. Streptococcus pyrogens
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6
Q

Cocci bacteria

A
  1. Staphylococcus aureus
  2. Staphylococcus oxalis
  3. Streptococcus pyogens
  4. Streptococcus pneumoniae
  5. Neisseria gonorrhoea
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7
Q

Rod shaped bacteria

A
  1. E. Coli
  2. Bacillus anthracis
  3. Corynebacterium diphtheria
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8
Q

Vibrio bacteria

A
  1. Vibro cholerae
  2. Vibro harveyi
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9
Q

Spirillum shaped bacteria

A
  1. Spirillum volutans
  2. Helicobacter pylori
  3. Campylobacter jejuni
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10
Q

Gram negative bacteria (KESPP)

A
  1. Pseudomonas
  2. Klebsiella
  3. Escherichia
  4. Salmonella
  5. Citrobacter
  6. Morganella
  7. Aeromonas
  8. Providencia
  9. Proteus
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11
Q

Gram positive (MNSSCA)

A
  1. Mycobacterium
  2. Norcardia
  3. Streptococci
  4. Staphylococci
  5. Clostridium
  6. Actinomyces
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12
Q

Sources of nitrogen

A

Peptone, meat, amino acid, yeast

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13
Q

Carbon

A

Organic acids and sources

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14
Q

Minerals

A

NaCl is used to provide isotonic cdts

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15
Q

Nutrient broth components

A
  1. Lab lemco powder - 1g
  2. Yeast extract - 2g
  3. Peptone - 5g
  4. NaCl - 5g
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16
Q

Nutrient broth total amount

A

13g in 1000ml

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17
Q

Lab lemco powder

A

It has the ability to support bacteria growth

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18
Q

Yeast extract

A

It’s a rich source of amino acids, vitamins and minerals

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19
Q

Peptone

A

Makes nitrogen available in the form of amino acids

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20
Q

NaCl

A

It maintains the osmotic balance of the medium

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21
Q

Other solidifying agents

A

Pectin, silica gel ,silicone, starch, Xanthan gum, gelatin

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22
Q

Nutrient agar total amount

A

28g

23
Q

Nutrient agar amount of water for dissolving

A

90ml

24
Q

Nutrient broth amount of water for dissolving

A

60ml

25
Q

Concentration of Agar

A

It is usually used in concentrations of 2- 3%

26
Q

Components of peptone

A
  1. Nicotinic acid
  2. Riboflavin
  3. Peptones
  4. Amino acids
  5. Inorganic salts ( magnesium, calcium)
27
Q

Disadvantages of liquid media

A
  1. Growth in liquid media can not be ordinarily quantitated
  2. Bacteria grown in liquid media form colloidal suspension
  3. Identification of cultures in liquid media requires sub growth onto a solid media.
28
Q

Examples of liquid media

A

Nutrient broth, peptone water, tryptone water, Koser’s citrate medium, MRVP Medium, sugar medium

29
Q

Examples of solid media

A
  1. Nutrient agar
  2. Blood agar
  3. MacConkey agar
  4. Mannitol salt agar
  5. Bismuth sulphite agar
  6. Cetrimide agar
  7. Chocolate agar.
30
Q

Semisolid agar

A

Prepared with the use of a reduced conc of agar ( 0.2- 0.4%)

31
Q

Examples of semisolid media

A
  1. Hugh and Leifson’s oxidation fermentation medium
  2. Stuart’s and Amies media
  3. Mannitol motility media
32
Q

MacConkey agar

A

For cultivation of gram-negative bacteria (KESPP)

33
Q

Blood agar

A

Can be used for fastidious organisms( mycobacterium tuberculosis)

34
Q

Simple media

A

Media that serve as the basal media for other media .Eg nutrient broth and nutrient agar.

35
Q

Complex media ( undefined media)

A

Media, which contains complex ingredients in unknown proportions

36
Q

Defined media

A

This is a media that contains defined proportions of all ingredients. Eg Dubos’ medium

37
Q

Special media

A
  1. Enriched media
  2. Enrichment media
  3. Selective media
  4. Differential media or indicator media
  5. Transport media
  6. Sugar media
38
Q

Enriched media

A

Mostly used for fastidious organisms.
Are prepared by adding substances like blood,serum, or egg to simple media. Egs blood agar, chocolate media

39
Q

Lactose fermenting bacteria like E. Coli

A

Form pink colonies on MacConkey agar

40
Q

Non lactose fermenting bacteria like Salmonella sp

A

Form pale or colourless colonies on MacConkey agar

41
Q

MacConkey agar

A

Inhibits Gram-positive cocci bacteria

42
Q

Transport media

A

They are used to ensure the viability of microorganisms in clinical specimens during their transport to the lab

43
Q

Transport media

A

They lack carbon, nitrogen, and other growth factors; hence, don’t encourage multiplication.
They contain only buffers and salt.

44
Q

Sugar media

A

Contains 1% of sugar( carbs)

45
Q

Exales of organisms that can test positive for hydrogen sulphide production test

A

E.Coli and Salmonella

46
Q

Examples of organisms that can test positive for citrate utilisation test

A

Klebsiella aerogenes,klebsiella pneumonia, and Proteus mirabillis

47
Q

Organisms that test negative for citrate utilisation

A

E.Coli and Shigella dysenteria

48
Q

Organisms that test positive for indole production (EPV)

A
  1. Escherichia coli
  2. Proteus vulgaris
  3. Vibrio cholerae
49
Q

Organisms that test negative for indole production test(SKP)

A

Salmonella typi
Klebsiella spp
P.mirabi

50
Q

Methyl red test

A

Used to distinguish between E. Coli(+ve) from Enterobacter(MR -ve).
And Yerisinia +ve from other gram- negative non-enteric bacteria ( MR -ve)

51
Q

Voges
P

A
52
Q

Cetrimide agar

A

It is selective for highly resistant bacteria like Pseudomonas aeruginosa and Mycobacterium tuberculosis.

53
Q

Cetrimide agar ( Pseudosel agar or pseudomonas cetrimide agar)

A

It inhibits E. Coli and other gram neg and pos bacteria

54
Q

Example of non-hemolytic bacteria

A

Vibrio cholerae

Micro Biology (7 decks)

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