LAB Flashcards
A positive urine urobilinogen test indicates all of the
following pathologic conditions except:
o Hemolytic anemia
o Obstructive jaundice (Tumor)
o Malaria
o Pernicious anemia
o Obstructive jaundice (Tumor)
Urobilinogen is a colorless bile pigment. Urobilin is
also a colorless pigment
o The first statement is true. Second statement is
false
o The first statement is false. Second statement is
true
o Both statements are false
o Both statements are true
The first statement is true. Second statement is
false
Which of the following causes the conversion of
urobilinogen in the intestinal tract from conjugated
bilirubin?
o Bacterial action
o Parasitic action
o Viral action
o Fungal action
o Bacterial action
When is the best time to collect urine for collection
of increased amount of urobilinogen in urine?
o Anytime of the day
o Early morning
o 2 hours after a meal
o 2-4 hours after 12 noon
o 2-4 hours after 12 noon
In which of the following conditions is urobilinogen
present in increased amount in urine?
I. Malaria
II. Pernicious anemia
III. Cholelithiasis
IV. Congestive Heart Failure
V. Nephrotic Disease
VI. Infectious Mononucleosis
o I,III,V,VI
o I, II, III, IV
o I, II, IV, VI
o II, III, V, VI
o I, II, IV, VI
Presence of urobilinogen in urine products which of
the following results with Ehrlich’s reagent?
o White precipitate
o Magenta color
o Greenish fluorescence
o Green to blue color
o Magenta color
Which two urine chemical tests provide helpful
information for differential diagnosis of hepatic
condition?
o Bilirubin and Urobilinogen
o Occult Blood and Bilirubin
o Urobilin and Bile acids
o Urobilinogen and Protein
o Bilirubin and Urobilinogen
The classic Ehrlich’s Reaction is based on the
reaction of urobilinogen with _______
o p-dichlorobenzene diazonium salt
o p-aminobenzoic acid
o diazotized dichloroaniline
o p-dimethylaminobenzaldehyde
o p-dimethylaminobenzaldehyde
Which of the following tests will not detect
presence of urobilinogen in urine?
o Schwartz-Watson’s Test
o Ehrlich’s Test
o Reagent Strip Test
o Pettenkofer’s Test
o Pettenkofer’s Test (BILE ACIDS)
The first step of Ehrlich’s test is to add 10 mL
Ehrlich’s reagent to 1 mL of urine. Development of
dark red color is the result that signify normal
amount of urobilinogen
o The first statement is true. Second statement is
false
o The first statement is false. Second statement is
true
o Both statements are false
o Both statements are true
o Both statements are false
10 ml urine and 1 ehrlich reagent
Pink- normal
What is the linear tetrapyrrole compound primarily
responsible for the yellow brown color of urine?
o Bilirubin
o Urobilin
o Melanin
o Stercobilin
o Urobilin
Which of the following tests would detect urobilin in
urine?
o Schlesinger’s Test
o Pettenkofer’s Test
o Benzidine Test
o Schwartz-Watson’s Test
o Schlesinger’s Test
Green fluorescence indicates a positive result for
Schlesinger’s test. No green fluorescence indicates a
negative result for the Schlesinger’s test
o The first statement is true. Second statement is
false
o The first statement is false. Second statement is
true
o Both statements are false
o Both statements are true
o Both statements are true
What liver function test is based upon the bile
pigment metabolism?
o Schlesinger test
o Salt acetic acid test
o Seliwanoff test
o Sulkowitch test
o Schlesinger test
What causes the oxidation of urobilinogen into
urobilin?
o Exposure to light and air
o Exposure to dark light and air
o Exposure to chemicals and heat
o Exposure to cold temperature and air
o Exposure to light and air
Which of the following test results with Iodine
indicates presence of increased amount of urobilin
in urine?
o White precipitate
o Greenish fluorescence
o Magenta color
o Green to blue color
o Greenish fluorescence
Which of the following substances includes all
others in the list?
o Porphobilinogen
o Bile pigments
o Urobilin
o Urobilinogen
o Bile pigments
Which of the following substances does not belong
to the group?
o Urobilinogen
o Stercobilin
o Urobilin
o Blood
o Blood
The first step of Schlesinger’s test is to mix 1 mL of
zinc acetate and 1 mL of alcohol in a test tube. The second step of Schlesinger’s test is to mix 1 mL of
urine specimen and 5 drops of iodine
o The first statement is true. Second statement is
false
o The first statement is false. Second statement is
true
o Both statements are false
o Both statements are true
o Both statements are true
Most of the urobilinogen in feces are oxidized to
stercobilinogen by intestinal bacteria which gives
stool their characteristic brown color. Some
urobilinogen is reabsorbed from the gut into the
portal blood and transported to the kidney, where it
is converted to yellow urobilin and excreted.
I. The first statement is true
II. The first statement is false
III. Second statement is false
IV. Second statement is true
o I & III
o I & IV
o II & III
o II & IV
o II & IV
The first step of Pettenkofer’s test is to pipet 3 mL of
urine in a test tube. The second step of
Pettenkofer’s test involves adding some sucrose
powder into the same test tube.
I. The first statement is true
II. The first statement is false
III. Second statement is false
IV. Second statement is true
o I & III
o I & IV
o II & III
o II & IV
o II & IV
3 mL (2mL urine)
Which of the following tests depends on the
reaction of bile acids and sucrose in acid solution to
form a red colored compound?
o Sulkowitch test
o Pettenkofer’s test
o Schlesinger test
o Ehrlich’s test
o Pettenkofer’s test
In testing urine for the presence of bile acids, why
do you have to cool the tube under running water
after the addition of concentrated H2SO4 to the
urine with sucrose solution?
o To avoid the rise of temperature to 90 degrees C
o To avoid the corrosive effect of H2SO4
o To avoid the rise of temperature to 70 deg C
o To provide proper temperature for the reaction
to take place
o To avoid the rise of temperature to 70 deg C
- Which of the following conditions is indicated by the
positive test for bile acids in urine?
I. Obstructive Jaundice
II. Hepatitis
III. Hepatocellular Jaundice
IV. Cholelithiasis
o 1 and 3 (x)
o 2 and 3
o 2 and 4
o 1 and 2
o 2 and 3
All of the following concentrations of sucrose
solution is NOT used to test for presence of bile
acids in urine EXCEPT:
o 50%
o 5%
o 15%
o 10%
o 5%
- Presence of which of the following substances in
urine would not interfere with the test for bile
acids?
o Chromogenic substances
o Blood (x)
o Urobilin
o Urobilinogen
o Chromogenic substances
How much urine specimen is required for
Pettenkofer’s test?
o 10 mL
o 2 mL
o 5 drops
o 5 mL
o 2 mL
The first step of Hay’s test is to pipet 3 mL of water
into two test tubes. One of those tubes will be
added with one of the bile salt solution
o The first statement is true. Second statement is
false
o The first statement is false. Second statement is
true
o Both statements are false
o Both statements are true
o Both statements are true
What is the acid used in Pettenkofer’s test?
I. Concentrated H2SO4
II. Concentrated HCl
III. Concentrated Acetic Acid
IV. Concentrated Ammonium Hydroxide
o II is correct
o II, III and IV are correct
o I is correct
o I and II are correct
I. Concentrated H2SO4
In the Pettenkofer’s test, 15% sucrose solution is
prepared by dissolving 5 grams of reagent sucrose in
water and diluting it to 100 mL. in the Hay’s test, the
bile salt solution is prepared by dissolving 10 grams
of sodium dechocolate in 100 mL water.
o The first statement is true. Second statement is
false
o The first statement is false. Second statement is
true
o Both statements are false
o Both statements are true
o Both statements are false
5% sucrose, 1 gram in 100 ml water
All of the following types of renal diseases yield
positive result with Benzidine test except:
1. Goodpasture disease
2. Diabetic nephropathy
3. Nephrotic syndrome
4. IgA nephropathy
o 1 and 4
o 1 and 2
o 2 and 3
o NOTA
o NOTA
Benzidine test could detect all of the following cells
and substances in urine except:
o Hemoglobin
o Myoglobin
o WBCs
o RBCs
o WBCs
Which of the following conditions refers to the
presence of intact red blood cells in urine?
o Hemoglobinuria
o Hematuria
o Pyuria
o Myoglobinuria
o Hematuria
Hematuria will give a speckled pattern in the blood
reagent strip. Myoglobin will also give a speckled
pattern in the blood reagent strip.
o The first statement is true. Second statement is
false
o The first statement is false. Second statement is
true
o Both statements are false
o Both statements are true
o The first statement is true. Second statement is
false
What happens when hydrogen peroxide is added to
a mixture of urine with presence of blood and
benzidine solution?
o Reduction of hydrogen peroxide to peroxidase
producing blue or green color
o Oxidation of benzidine due to peroxidase
released by heme
o Production of blue or green color due to action
of benzidine to the hemoglobin
o Decomposition of hydrogen
o Oxidation of benzidine due to peroxidase
released by heme
Within 10 minutes, obtaining a blue or pink color in
Benzidine test indicates presence of blood. Blue
color developing after 10 minutes is not a positive
test but is due to the oxidation of benzidine to
atmospheric oxygen
o The first statement is true. Second statement is
false
o The first statement is false. Second statement
is true
o Both statements are false
o Both statements are true
o The first statement is false. Second statement
is true
The first step of Benzidine test is to mix 3 mL of
saturated benzidine solution and 3 mL of urine. The
second step of Benzidine solution is to add 1 mL of
hydrogen peroxide to the benzidine urine mixture
o The first statement is true. Second statement is
false
o The first statement is false. Second statement
is true
o Both statements are false
o The first statement is false. Second statement
is true
All of the following substances would cause false
negative result for blood if present in urine except:
o Oxalates
o Urates
o Sulfates
o Ascorbic acid
o Oxalates
The principle for the test for blood in urine is based
on the peroxidase-like activity of the _______ which
decomposes hydrogen peroxide with the release of
_________ that oxidizes benzidine producing a
colored compound.
o Globin portion of hemoglobin – O2
o Heme portion of hemoglobin – O2
o Heme portion of hemoglobin – CO2
o Globin Portion of Hemoglobin – CO2
o Heme portion of hemoglobin – O2
A positive benzidine test indicates all of the
following conditions except:
o Hemoglobinuria
o Pyuria
o Myoglobinuria
o Hematuria
o Pyuria
Which of the following renal disorders is indicated
by a positive Sulkowitch Test?
1. Osteoporosis
2. Hyperthyroidism
3. Renal tubular acidosis
4. Renal lithiasis
o 1, 2 and 3
o 1, 2, 3 and 4
o 2 and 3
o 1 and 2
o 1, 2, 3 and 4
Michelle, an old lady complained of burning pain in
the pelvic area with difficulty in urinating. Initial
diagnosis is kidney stones. The physician requested
for a Sulkowitch test and the result was positive.
What crystal might be the cause of discomfort to
patient Michelle?
o Uric acid
o Calcium carbonate
o Cystine
o Calcium oxalate dehydrate
o Calcium oxalate dehydrate
How much Sulkowitch reagent was added to a
specified volume of urine?
o 3 mL
o 2 mL
o 5 mL
o 10 mL
o 3 mL
- Based on what you had learned, what were those
white precipitates formed when urine was mixed
with Sulkowitch reagent?
o Calcium carbonate
o Ammonium magnesium phosphates
o Calcium oxalates
o Calcium phosphates
o Calcium oxalates