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BIO 133 Lab > Lab #1 blood and hematology > Flashcards

Lab #1 blood and hematology Flashcards

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Biology 101 flashcards
1
Q

What kind of tissue is blood

A

Connective tissue

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2
Q

What percentage of blood volume is plasma

A

55%

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3
Q

What kind of dissolved molecules does plasma contain

A

Electrolytes, plasma proteins, nitrogenous waste products, nutrients (glucose fatty acids) gasses, and hormones

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4
Q

What is hematocrit/packed cell volume

A

when you compare the volume of the packed cells (red blood cells) with the volume of plasma and white blood cells after spinning it in a centrifuge

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5
Q

How would you determine grams of hemoglobin per decaliter from a hematocrit

A

would divide the hematocrit by 3 ie if there was 45% PCV then hemoglobin would be 15 gHb/dl

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6
Q

if there was 50 cells counted on a hemocytometer what would the red blood cell count be if the dilution factor was 200

A

(number of cells counted x Dilution factor) / Volume of blood that was used

(50 x 200) / 0.02 mm cubed of blood

the 0.02 mm cubed because the dimensions of each square on a hemocytometer 0.2mmx0.2mmx0.1mm which equals 0.004 and then there are 5 of them all together so 0.004x5= 0.02 mm cubed
so there would be 500,000 red blood cells per mm cubed of blood

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7
Q

What are two illnesses related to red blood cell counts

A

Anemia
polycythemia

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8
Q

What is anemia

A

low oxygen carrying capacity of blood could be due to low red blood cell count overall or low hemoglobin in the blood low blood cell count results in this condition

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9
Q

what is polycythemia

A

Higher than normal red blood cell counts

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10
Q

What is agglutination

A

when antigen and antibody clump together and it usually results in the destruction of the foreign material

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11
Q

What is an antigen

A

antigen would be the indicator glycoprotein that is embedded in the cell as a marker ie the thing embedded in the red blood cell that lets the body know its not foreign

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12
Q

What is an antibody

A

the thing that circulates in the blood and attaches to cells if it detects a foreign antigen and destroys it

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13
Q

what is erythroblastosis fetalis

A

when the mother and the baby have different RH blood types

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14
Q

What are the two groups of white blood cells

A

Granulocytes and agranulocytes

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15
Q

What are the types of granulocytes

A

Remember NEB
Neutrophils
Eosinophils
Basophils

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16
Q

What are the types of agranulocytes

A

Lymphocytes
Monocytes

17
Q

What is the role of Neutrophils and how could you identify them

A

Their job is to phagocytize (eat) bacteria
they have multi lobed nucleus and red and blue cytoplasmic granules

18
Q

What is the role of Eosinophils and how could you identify them

A

Defend body against parasitic worms and have a role in the allergic response
they have a bi lobed nucleus and red cytoplasmic granules

19
Q

what is the role of basophils

A

Produce histamine to initiate the inflammatory response
Have a bilobed nucleus and purplish black cytoplasmic granules

20
Q

what is the role of lymphocytes and how to identify them

A

T cells bind and recognize virus infected or tumor cells and B cells produce antibodies
very large nucleus that almost takes up the entire cell

21
Q

What’s the role of monocytes and how to identify them

A

Can become macrophages to phagocytize foreign objects
have a large kidney bean shaped nucleus

22
Q

What is the equation for white blood cell count

A

(number of cells counted x 200) / 0.4 mm cubed

23
Q

what is leukocytosis

A

When an individual has a high WBC count

24
Q

What is leukopenia

A

when white blood cell count is very low

25
Q

What is neutrophilia and what could it indicate

A

High neutrophilia counts
could indicate local infection

26
Q

What is Neutropenia and what could it indicate

A

Low count of neutrophils
Occurs when you have a virus

27
Q

What is Eosinophilia and what could it indicate

A

High eosinophil count
could indicate parasitic worm or allergies

28
Q

What is lymphocytosis and what could it indicate

A

High lymphocyte counts could indicate viral infection

29
Q

What blood cell is this and what does it do what color are its granules

A

Basophil
initiates inflammatory response
purple black granules

30
Q

What blood cell is this and what does it do what color are its granules

A

Eosinophils
red granules
Protects against worms

31
Q

What blood cell is this and what does it do what color are its granules

A

Lymphocyte
Binds to and recognizes viruses or tumor cells
no granules

32
Q

What blood cell is this and what does it do what color are its granules

A

Monocyte
can become macrophage to eat foreign objects

33
Q

What blood cell is this and what does it do what color are its granules

A

Neutrophils
Phagocytizes bacteria
red blue granules

BIO 133 Lab (8 decks)

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