Lab 1 - Assaying Biomolecules Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

What is usually quantified when a standard curve (calibration curve) is generated?

A

the amount or concentration of an analyte in a sample

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Which relationship does a standard curve describe?

A

the relationship between signal intensity and amount/concentration of the analyte

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What are the two steps that lead to color developpment because of RC DC?

A

1- Reaction between the copper and protein at alkaline (basic) pH
2- The reagent of the Folin agent by the copper-treated protein.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Why is the RC DC assay coloremetric?

A

AS the concentration of the protein goes up, the color of the samples become darker.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the purpose of using a control?

A

It generates data to which the experimental data can be compared. In th case of this lab, it creates a standard curve to which can be extrapolated unknown concentrations. The buffer calibrates the spectrophotometer, so all the data is relative to the buffer.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly