Lab #1 and Rules and Regulations

Question 1

Where do we keep cultures and why?

Answer 1

In test tubes to prevent accidents and to avoid contamination of yourself and the environment.

Question 2

What do we do when we spill cultures?

Answer 2

Cover with paper towels and saturate them with germicide for 15 minutes then dispose of it.

Question 3

When handling contaminated material:

Answer 3

Don’t place contaminated instruments on bench tops, contaminated pipettes and cotton swabs must be disposed in biohazard container, never pipette by mouth, contaminated test tubes must be placed in wire basket in the autoclave bin, live mounts of hazardous organisms must be soaked in germicide for 15 minutes before washing with soap and water.

Question 4

When using Bacti-cinerators:

Answer 4

Optimum sterilizing temperature (150F/15C) after 10 minutes. Don’t leave inoculating loops or needles in Bacti-cinerator unattended.

Question 5

Labeling

Answer 5

Use wax pencils on test tubes and microscope slides.Permanent markers can be used on Petri plates.

Question 6

Who introduced the world to the existence of microbial forms of life?

Answer 6

Anton Van Leeuwenhoek

Question 7

Microscopes are designated as either ___ ________ or ___________ ___________.

Answer 7

light microscopes, electron microscopes

Question 8

Flourescent microscopes

Answer 8

Use ultraviolet radiation whose wavelengths are shorter than those of visible light and are not directly perceptible to the human eye.

Question 9

Electron microscopes

Answer 9

Use electron beams (instead of light rays) and magnets (instead of lenses) to observe submicroscopic particles. Magnification power of up to 1 million x.

Question 10

Brightfield microscope

Answer 10

Contains two-lens systems for magnifying specimens: the ocular lens in the eye piece and the objective lens located in the nosepiece. Specimen is illuminated by light focused on it by substage (condenser).

Question 11

What is the major limitation of a Brightfield microscope?

Answer 11

The absence of contrast between the specimen and the surrounding medium, which makes it difficult to observe living cells.

Question 12

1 kilogram = ___ lbs (pounds)

Answer 12

2.2 lbs

Question 13

1 ounce (weight) = ___ grams

Answer 13

28.4 grams

Question 14

1 fluid ounce (volume) = ____ ml

Answer 14

29.6 ml

Question 15

1 quart = ___ ml

Answer 15

946 ml

Question 16

1 inch = ____ cm

Answer 16

2.54 cm