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Physiolab > Lab 1 > Flashcards

Lab 1 Flashcards

1
Q

Effects of heparin?

A

prevents blood from coagulating by catalysing the thrombin-antithrombin reaction

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2
Q

What is the function of methanol fixation?

A

fix the smear and precipitate the proteins

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3
Q

Buffy coat, explain

A

White blood cells have lower density than red blood cells, and will fom a thin layer on the top after centrifugation

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4
Q

How to obtain blood serum

A

Centrifugate blood samle where fibrinogen is changed into fibrin by shaking flask containing small glass beads

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5
Q

Defibrilated blood

A

shake flask with blood and small glass beads to change fibrinogen into fibrin

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6
Q

What happens with the hematocrit level while increasing epinephrine level?

A

increase

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7
Q

Anticoagulants, give examples

A

Sodium citrate and Ethylene Diamine Tetraacetic acid (EDTA)

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8
Q

Lee White method

A

tilt blood in tube in intervals for 3 min to see if it coagulates

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9
Q

Definition of hematocrit

A

Ratio of red blood cells to the total blood volume

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10
Q

Venous hematocrit larger than arterial, explain

A

because of ion transport and water flux associated with the CO2 transport

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11
Q

Increased eruthrocyte sedimentation rate

A

By displacing the albumin, the surface decrease

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12
Q

Describe the neutrophil granulocytes (mammals)

A

-nucleus: bean shaped (young), elongated (middleaged),
segmented (mature)
-light pink cytoplasm, different granules
-10qm diameter
-enzymes: lysozyme, myeloperoxidase

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13
Q

Describe heterophil granulocyte (birds)

A
  • nucleus: several segments
  • pink cytoplasm
  • pale red granules
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14
Q

Describe eosinophil granulocyes

A
  • nucleus: segmenter (unually bi-lobed)
  • large bright red granules
  • 14qm diameter
  • Enzymes: histaminase, acid & alkalone phosphatase, eosinophil basic & cationic protein
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15
Q

Describe basophil granulocyte

A
  • nucleus: hardly segmented
  • large blur granules
  • 15qm diameter
  • enzymes: protease
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Physiolab (5 decks)

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