Lab 1 & 2 Quiz Flashcards
What are the three general rules when using the compound microscope?
- Do not remove any parts of the microscope
- Clean the lenses daily
- Do not touch the glass surfaces of the lenses with your fingers
Where are the oculars?
The two tubes you look through
Where is the interpupillary distance scale?
The scale on the binocular tube that separates the oculars
Where is the binocular tube?
The neck like structure that connects the oculars to the arm
Where is the revolving nosepiece?
It is the thing that holds the three objective lenses and is connected to the arm
Where are the mechanical stage controls?
Vertical knob in front of the focus controls
Where is the condenser and iris diaphragm?
Right under the stage
Changes how much light goes through
Where is the iris diaphragm adjustment lever
A little stick poking out the iris diaphragm
Condenser adjustment knob
Left side of the microscope right behind condenser
What is a fluorescence microscope?
Absorbs one particular wavelength of light and emits some of that absorbed light at another wavelength
Black background with some colour (kinda 3D)
Uses dye
Eat dye and take x ray
What is confocal laser scanning microscopy?
Focuses a laser light at a very thin plane
Looks infrared
Reconstructed 3D image
What is transmission electron microscopy?
Transmits a beam of electrons through a thin section of a specimen
Best resolution of internal organization of cells and organelles
Black and white, can’t see outside the cell
Electrons going through
What is scanning electron microscopy
A beam of electrons is scanned over the surface of a specimen resulting in a 3D image of the surface
Used for intact specimen
Colour depends on what you coat item with
Electrons bouncing back
What is a frequency?
The proportion of one type of thing to the total population
What is a resolution?
The ability to detect or distinguish structures that are very close together
