lab 1

Question 1

Image taken with the aid of a microscope

Answer 1

micrograph

Question 2

A device that uses two sets of lenses
to increase the apparent size of
objects

Answer 2

compound microscope

Question 3

● The scale of enlargement of an image
● Total magnification is defined by:
ocular x objectives

Answer 3

magnification

Question 4

● The ability of a microscope to
distinguish fine details within an object

Answer 4

resolving power

Question 5

The shortest distance between two points that can still be visually distinguished as separate

Answer 5

resolution

Question 6

parts of a microscope (mechanical)

Answer 6

a. Body tube / Draw tube
b. Arm
c. Base
d. Stage
e. Stage clips
f. Iris diaphragm
g. Light Source / Mirror
h. Revolving nosepiece
i. Adjustment knobs
i. Coarse adjustment knob
ii. Fine adjustment knob

Question 7

parts of a microscope (optical)

Answer 7

a. Eyepiece / Ocular lens
b. Scanning Objective Lens
c. Low Power Objective (LPO)
d. High Power Objective (HPO)
e. Oil Immersion Objective (OIO)

Question 8

• Also known as stereomicroscope
• 10x - 40x common range of
  magnification

Answer 8

dissecting microscope

Question 9

The distance between the front edge of the objective lens and the specimen surface (with the surface of the cover glass in case of the cover glass objective lens) when the specimen is focused.

Answer 9

working distance

Question 10

● The maximum area of the sample visible
when looking through the microscope
eyepiece
● The higher the magnification, the smaller
the microscope field of view will be

Answer 10

Field of View

Question 11

● Also called as depth of focus
● The depth of the specimen layer which is
in sharp focus at the same time
● Switching to higher power reduces the
____

Answer 11

Depth of Field

Question 12

● A measure of its ability to gather light and
resolve fine specimen detail at a fixed
object distance.

Answer 12

Numerical Aperture