Lab 1 Flashcards
What size are the 3 objective lenses
4x 10x 40x
Iris Diaphragm changes ___________
resolution
Size of organism calculation
= (objective lense diameter)/(times organism can fit across)
Prokaryotic
no membrane bound organelles
circular nucleoid
Eukaryotic
membrane bound organelles
larger than prokaryotic
Cyanobacteria
blue-green bacteria containing pigment phycocyanin
Frequency of genus calculation
in sample/ total
Relative total pollution index (rTPI) calculation
Pollution x frequency
Which number should you look at when finding if an environment has organic pollution
Total pollution index
Fluorescence Microscopy
absorbing one particular wavelength and emitting some of that absorbed light. Dyes added to label specific molecules
Confocal Laser Scanning Microscopy
focusing light on very thin planes. used in specimens too thick for fluorescence. can be rearranged into 3D
Electron Microscopy benefits
greater resolving power
Transmission electron microscopy
beam of electrons passing through thin sections. best resolution is best for internal stuctures
Scanning electron microscopy
beam of electrons scanned over specimen surface. 3D image
Condenser
focuses light at the level of a specimin
