Lab #1

Question 1

What is the most common light source for brightfield microscopy? 

Answer 1

Tungsten-halogen lamp

Usually uses a blue filter To reduce yellow output from lamp

Question 2

This is located at the light source exit,

Controls the diameter of light beam, And reduce the Straylight from the light source

Answer 2

Felid diaphragm

Question 3

Field diaphragms are usually an ________ diaphragm 

Answer 3

Iris

Question 4

• This evenly distributes and concentrates light into specimen
• two lenses
• Aperture diaphragm

Answer 4

Condenser

Question 5

• Regulates angle of beam of light presented to specimen

- adjusted with an Iris or disc diaphragm

Answer 5

Aperture diaphragm

Question 6

Proper adjustment of the __________ and ___________ gives maximum resolution, contrast, and definition

Answer 6

Condenser, aperture diaphragm

Question 7

What is the most important optical component of the microscope?

Answer 7

Objective lenses

• it produces the primary image magnification

Question 8

Higher magnification objectives have ____________ Working distances

Answer 8

Shorter

A 10X objective ad a distance of 7.2 mm
A 40X objective has a distance of 0.6 mm
* exercise care when focusing high power objectives

Question 9

A measure of the resolving power of the lens

Answer 9

Numerical aperture

Question 10

What are the objective magnifications?

Answer 10

The eyepiece is 10X for all…
•Scanning (red) —- 4X ——40X total 
•Low power (yellow) —10X—100X total
•High  “dry” power (blue)—40X—400 total 
•oil immersion (white)—100X—1000X total

Question 11

All Clinical microscopes have what type of eyepiece?

Answer 11

Binocular

• One eyepiece has an adjustable diopter