L9- Microbial associations with plants Flashcards

1
Q

What’s in a nitrogenase complex?

A
  • Dinitrogenase- Fe and Mo cofactors

- Dinitrogenase reductase- Fe cofactor

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2
Q

Where do nitrogenase complexes occur?

A

Free living aerobes
Free living anaerobes
Symbiotic microbes with plants

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3
Q

What do legumes have on their roots?

A

Specialised organs called nodules, containing bacteria called rhizobia

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4
Q

Where do Rhizobia invade the roots?

A

Either within the nodule or in the soil, but only fix N2 inside the nodule

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5
Q

How do Rhizobia invade the roots?

A
  1. Recognition and attachment at root hair
  2. Excretion of nod factors cause root hair curling
  3. Rhizobia penetrate root hair and multiply within an infection thread
  4. Bacteria in infection thread grow towards root cell
  5. Formation of bacterioid state within the plant cell
  6. Continued plant and bacterial cell division
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6
Q

What do Rhizobia in the plant cells differentiate into?

A

N2- fixing cells called bacteroids

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7
Q

In a mutualistic relationship, what does the plant gain? What does the bacteria gain?

A

Plant- nitrogen

Bacteria- anaerobic environment for N2 fixation and carbon for energy

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8
Q

What are Mycorrhizal associations?

A

Fungal associations with plant roots

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9
Q

Where does it occur?

A

In 95% of all vascular plants

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10
Q

What are the 2 main types of Mycorrhizal associations?

A
  1. Ectomycorrhiza- mainly basidiomycetes/trees

2. Endomycorrhiza- mainly zygomycetes/grasses

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11
Q

In ectomycorrhiza, what effect does it have?

A

No plant cell invasion

Greatly increases effective root surface area

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12
Q

What does the Mycorrhizal fungus do?

A

Uptake of inorganic nutrients, particularly phosphate

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13
Q

What does the tree provide for mycorrhizal fungus?

A

Sugars from photosynthesis

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14
Q

What is AM?

A

Arbuscular mycorrhiza fungus-> where the fungus penetrates the corticol cells of the roots of a vascular plant.

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15
Q

Which group is AM in?

A

Endomycorrhiza

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16
Q

What happens in the plant when AM invade?

A

Hyphae penetrate cells- arbuscles. Form branched clusters of hyphae, nutrient exchange, large surface area.

17
Q

Why do orchids rely on mycorrhiza?

A

They initially lack chlorophyll and some even lack it as adults

18
Q

What do AM fungi produce within the roots?

A

Vesicles containing lipids, used for storage

19
Q

What happens when orchid cells are invaded?

A

Hyphae form coils called pelotons. Pelotons incrase surface area between orchid and fungi. Each peloton has a short life span and then is digested by the orchid cell.

20
Q

Relationships between orchids and mycorrhiza are unstable. What are the 3 potential infection outcomes?

A
  1. Germination and mycorrhiza formation
  2. Parasitisation, orchid dies
  3. Orchid rejects infection, orchid dies
21
Q

What are lichens?

A

Organism made of cyanobacteria or algae (or both) living among filaments of a fungus in a mutually beneficial relationship.

22
Q

What are the layers of lichen?

A

Cortex (top)
Algal layer
Medilla (bottom)

23
Q

What do alga provide? WHat does the fungus provide?

A

Alga- provides sugars

Fungus- protects alga from desiccation

24
Q

Why can lichens be used as indicators?

A

They’re very sensitive to air pollution