L20- Microbial pathogens of plants and insects Flashcards

1
Q

To cause disease what 5 things must a pathogen do?

A
  1. Recognize the host,
  2. penetrate host barriers
  3. move through host tissue
  4. utilize host components (nutrition for growth and reproduction
  5. suppress host defence systems
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2
Q

What caused the potato famine?

A

Phytophtora infestans

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3
Q

What do the enzymes produced by the pathogen do?

A
  1. gain access to the host, disintegrate plant structure
  2. movement of microbe in host tissue
  3. impair cell function
  4. breakdown host molecules to absorb them
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4
Q

What do toxins made by pathogens do?

A
  1. disrupt host metabolism and kill cells

2. interfere with host cell functions

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5
Q

What do growth regulators made by the pathogen do?

A

alter the amount and activities of growth regulatory substances in plants to favour pathogen invasion

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6
Q

What are mos plant viruses transmitted through?

A

Insects (carried through grafting)

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7
Q

What is tristeza of citrus fruits?

A

Virus- caused. Causes declin in growth and lower yields. Severe outbreaks can kill citrus trees. loss of chlorophyll i think.

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8
Q

Give 5 plant bacterial diseases?

A
  1. soft rots- erwinia on vegetables
  2. wilts- clog vascular tissue- pierc’es disease of grapes
  3. blights- rapid destriction of plant tissue. Erwinia of apples and pears.
  4. Cankers (sores)- citrus canker
  5. Plant tumours- crown gall (transfer plasmid)
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9
Q

What are nectotrophs?

A

Fungal pathogens. Invade and kill plant tissue rapidly. Then live saprophytically on dead remains

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10
Q

How do nectotrophs penetrate the plant?

A

They penetrate directly by clinging on and puncturing the cell. End of germ tube anchors to leaf surface.
2. Germ tube swells, increase in internal turgor pressure.
3. thin infection peg “punches” through plant cell wall, using enzymes too,
This is called appressoria formation

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11
Q

What are biotrophs?

A

Fungal pathogens. Found on living plants. Have very complex nutrient requirements and do not kill host cells rapidly.

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12
Q

Give an example of a biotroph?

A

Powdery mildews and rusts. (pathogens are specialized for each plant)

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13
Q

What do necrotrophs and biotrophs form to invade plants?

A

Necrotrophs- appressoria

Biotrophs- haustoria

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14
Q

How do biotrophs invade plant hosts?

A

Produce haustoria. Punches into plant cell but doesn’t penetrate the cytoplasm so the plant can;t detect that it’s being penetrated.

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15
Q

Why does a plant carry on photosynthesising when its invaded by a biotroph?

A

It causes patches on the leaf but the rest of the leaf can carry on photosynthesising and providing sugars for the biotroph. The plant can;t detect the invasion so isn;t defending itself against the pathogen.

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16
Q

which pathogen causes disease in flour moths?

A

Bacterial disease- Bacillus thuringiensis. produces bt toxin as it multiplies in the inject lymph. Began to be used against lepidoptora (moths and butterflies) as pest control in 1950s.

17
Q

How was Bt used against root weevil larvae and pupae?

A

It’s highly insoluble so harmless to humans and animals.
Soluble in pH above 9.5, found in larvae lepidopteran guts.
So it’s very specific insecticidal agent.
Bacteria invades the host causing lethal septicaemia.

18
Q

How has Bacillus Thuringiensis been used in plants?

A

It genetically engineered crops. Bt crops.

19
Q

What are the 2 main categories of fungi that kill insects and nematodes?

A

1/ enteromophthorales

2. nematode-trapping fungi

20
Q

What are entomophthorales?

A

Group of fungi that invade and kill a range of insects
often host-specific but can kill related insects
Spores applied as a spray germinate, invade and kill insects

21
Q

What are nematode- trapping fungi?

A

Fungi that can form traps when nitrogen levels are low
Nematodes can cause problems, attackin crops or llivestock
used as a biocontrol agent for crops and cattle
quickly responding rings, contract when nematode worm goes though a ring of the fungi