L8 Binding Of Substrates, States And Mechanism

Question 1

What does the maximal velocity on a substrate velocity graph mean?

Answer 1

That’s when all enzyme active site are filled with substrate

Question 2

How do you calculate Km?

Answer 2

1/2 Vmax

Question 3

What do enzymes actually do to make a reaction faster?

Answer 3

Brings substrates closer together

Hold them in the optimal orientation so they react with each other

Question 4

In going from substrate to product, all reactions pass through what?

Answer 4

They pass through transition states and sometimes through intermediates

Question 5

Which is more stable, reaction intermediate or transition states?

Answer 5

Intermediate

Question 6

Do enzymes bind to their substrates tightly?

Answer 6

No, enzymes don’t bind their substrates too tightly then hypothetically the energy jump in activation energy would be the same as a non enzymatic reaction

Question 7

What are the 3 catalytic mechanisms?

Answer 7

1. General acid- base catalysis
2. Covalent catalysis
3. Use of metal ions in catalysis