L3 - Intracellular immunity (EBV) Flashcards
What are diseases are EBV associated with?
8 diseases (ppt slides)
Infectious Mononucleosis
Burkitt’s Lymphoma
What are facts about EBV?
co-evolution with humans
replication cycle - enter memory B cells, lytically replicate, remain latent
What is primary EBV infection WITH containment?
1 - asymptomatic infection
2 - Acute IM
3 - Recurrent infection
What is primary EBV infection WITH LOSS of containment (not mounting normal immune response)
1 - chronic active infection
2 - lymphoproliferative disorders
3 - malignancy
What is acute IM?
- infects 95% in childhood
- lymphoproliferative disorder
What is primary infection of EBV?
transmitted by direct binding/transfer from infected B cell
What are the first responders to EBV?
innate immune cells
drawn by danger molecules
What cells kill EBV infected cells?
CD8 cytotoxic cells
How are anti-EBV CD8 cells activated?
lytic antigens - 50% T cells activated
latent antigens - 1-5%
What does EBV mimic?
B cell activation
trick to it becoming a long-life memory-type cell
BCR and CD40 proteins are expressed as part of latency programme - these proteins drive differentiation of B cells
What are cancers associated with EBV?
Burkitt’s Lymphoma
Hodgkin’s Lymphoma
Nasopharyngeal Carcinoma
What is Burkitt’s Lymphoma?
aggressive
extranodul masses
Latency EBV expression programmes
EBNA-1 - oncogenesis
EBERs - inhibit apoptosis
How is EBV involved in BL?
genetic rearrangement to create growth-inducing hybrids of oncogenes
EBV represses apoptosis to make sure cell survives - becomes cancerous
What is XLP?
males only
invasion of bone marrow & liver
hepatitis and bone marrow hypoplasia
XLP encodes signalling protein with SH2 domains (SAP)- neg regulate T/NK cell signalling
