(L14) Cell Cycle, Cancer, and Cell Death Flashcards
What are the molecular regulators of the cell cycle? L14 S15
Cyclins:
-levels increase or decrease during certain times of cell cycle indicating the current phase
Cyclin dependent kinase (CDK):
- kinases that are active when bound to their specific cyclin
- kinase activity regulates relevant enzymes for the current phase of cell cycle
Cyclin dependent kinase inhibitor (CKI):
-inhibit CDKs
How are CDKs regulated?
L14 S18
Requires cyclin binding
Three phosphorylation sites:
- 1 activating (T-loop)
- 2 inactivating
What are the main cyclins?
L14 S17
- A
- B
- D
- E
What are the major inhibitory regulators of the cell cycle?
L14 S22
p21:
-adds inhibitory phosphate to CDKs
p27
p16/INK4
What events trigger entry into cell cycle?
What is the transcripition factor for this and what does it stiumulate?
L14 S23
Mitogen binds receptor which activates Ras by replacing GDP with GTP.
Ras-GTP activates RAF, RAF phosphorylates MEK, MEK phosphorylates MAP, and MAP phosphorylates Myc.
Myc is a transcription factor that:
- stimulate cyclin D transcription
- stimulates p27 degradation
- stimulates E2F transcription
What is the R point in the cell cycle?
L14 S25
Restriction point or G1 checkpoint
It occurs during late G1 and after the cell proceeds through, it is commited to dividing.
What cyclin/CDK complex allows for progression through the R point?
What is the mechanism?
L14 S17;25
Cylcin D-CDK4/6
Hypophosphorylates Rb (retinoblastoma protien) which inhibits E2F
What cyclin/CDK complex allows for progression through the G1 ► S checkpoint?
What is the mechanism?
L14 S17;24
Cylcin E-CDK2
Hyperphosphorylates pRb causing it to release E2F. E2F causes transcription of more cylin E and E2F, amplifying the process.
Initiates assembly of pre-replicaiton complex.
What cyclin/CDK complex allows progression through S phase?
What is the mechanism?
L14 S17;27
Cyclin A-CDK2
Stabilizes replicaiton complex.
What cyclin/CDK complex allows progression from S ► G2?
What does this compelx do?
L14 S17;25
Cyclin A-CDK1
Stimulates production of cyclin B.
What cyclin/CDK complex allows progression from G2 ► M?
What is the mechanism?
L14 S17;28
Cyclin B-CDK1
Activates CDC25 phosphorylase which activates more CDK1.
Initiates mitotic spindle assembly.
What is the function of CDC25?
L14 S19;28;30
Phosphorylase that removes inhibiting phosphates from CDKs.
What are ATM and ATR?
What pathways do they activate?
L14 S29-30;32
Both are kinases that are activated when DNA damge is detected.
p53 pathway:
- ATM/ATR phosphorylates Mdm2 causing it to release p53
- p53 activates p21
- p21 places inhibitory phosphate on CDKs
Chk pathway:
- ATM/ATR phosphorylate Chk, activating it
- Chk phosphorylates CDC25, inactivating it
- without CDC25, inhibitory phosphates are not removed from CDK
Differentiate between the terms tumor, neoplasm, and cancer.
L14 S35
Tumor:
-space occupying lesion
Neoplasm:
-abnormal growth caused by abnormal gene regulation
Cancer:
-malignant neoplasm
What are the stages of carcinogenesis?
L14 S38-42
Initiation:
- irreverisble change in DNA sequence
- activation of oncogene or inactivation of tumor supressor gene
Promotion:
- reversible gene activtion or repression
- cellular selection leads to clonal expansion
- once threshold is reached, cancer occurs
Progression:
- it is now cancer
- continued evolution of cancer for optimal growth