L13 Membrane Contact Sites

Question 1

FUSION DOES NOT OCCUR WHEN THERE IS TRANSPORT OF MATERIAL IN TERMS OF MEMBRANE CONTACT SITES.
TRUE OR FALSE

Answer 1

TRUE

Question 2

WHAT OCCURS AT RER

Answer 2

PROTEIN SYNTHESIS

Question 3

WHAT OCCURS AT SER

Answer 3

SITE OF LIPID SYNTHESIS

ROLES IN CA STORAGE

Question 4

WHAT CARRIES OUT REUPTAKE OF CALCIUM AFTER CONTRACTION

Answer 4

MEMBRANE CONTACT SITES

Question 5

WHAT IS THE PROCESS OF MEMBRANE CONTACT SITES AFTER CONTRACTION

Answer 5

1. POST CONTRACTION CA STORES ARE DEPLETED
2. DEPLETION CAUSES A CONFORMATIONAL CHANGE IN STIM1, A CA SENSOR IN THE ER TO BECOME MORE +VE
3. +VE CHARGES INTERACT WITH PIP2 ON THE PLASMA MEMBRANE ENABLING INTERACTION WITH ORAI1 CHANNEL ON THE PLASMA MEMBRANE
4. UNDER THESE CONDITIONS ORAI1 CAN OPEN TO LET CA DIRECTION BACK INTO THE LUMEN OF THE ER

Question 6

THE ………. MAKES CLOSE CONTACT WITH MANY DIFFERENT ……………………….. IN THE CELL BUT THERE IS NO …………………….. OF MEMBRANE INSERTION OR ……………………

Answer 6

ER
ORGANELLES
EVIDENCE
FUSION

Question 7

MUTATIONS IN MEMBRANE CONTACT SITES GIVE RISE TO

Answer 7

DISEASE

Question 8

ADVANCES IN …………………….. AND LIVE CELL …………………… HAVE ALLOWED UNDERSTANDING OF MEMBRANE CONTACT SITE ……………………. AND FUNCTION

Answer 8

MICROSCOPY
IMAGING
STRUCTURE

Question 9

WHAT IS THE STRUCTURE OF A MEMBRANE CONTACT SITE

Answer 9

SMALL SIZE BETWEEN MEMRBANES
NO RIBOSOMES PRESESNT
ER CONTACTS LONG LIVED

Question 10

WHAT ARE THE FUNCTIONS OF MEMBRANE CONTACT SITES

Answer 10

CA MOBILISATION
LIPID TRANSFER
SIGNALLING

Question 11

THERE ARE USUALLY ………………. CONTACT SITES. THE PROTEINS INVOLVED OFTEN HAVE ……………………… CONFORMATIONS TO ALLOW …………………. TO FORM ACROSS THE GAP BETWEEN …………………………….

Answer 11

MULTIPLE
EXTENDED
BRIDGES
MEMBRANES

Question 12

WHAT IS CLEM

Answer 12

CORRELATIVE LIGHT AND ELECTRON MICROSCOPY

Question 13

WHAT IS THE ROLE OF CLEM

Answer 13

ALLOWS LOCALISATION OF FLUORESCENTLY LABELLED PROTEIN TO MCS

Question 14

MCS ARE ALSO IMPORTANT FOR ………………… TRANSFER - NON VESICULAR TRANSFER. LIPID TRANSFER IS …………………….. BUT IT IS NOT UNDERSTOOD HOW.

Answer 14

LIPID

UNIDIRECTIONAL

Question 15

WHAT ARE THE PROTEINS IMPORTANT IN THE ENDOSOME FOR DELIVERY OF CHOLESTEROL TO THE ER

Answer 15

NPC1 AND NPC2

THESE INTERACT WITH ORP ON THE ER MEMBRANE

Question 16

WHAT DO MUTATIONS IN NPC1 AND NPC2 CAUSE

Answer 16

NIEMAN PICK DISEASE C

A PROGRESSIVE DISORDER DUE TO INABILITY OF TRANSPORT OF CHOLESTEROL TO THE CELL MEMBRANE

Question 17

MEMBRANE CONTACT SITES ARE INVOLVED IN ORGANELLE ………………..
MITOCHONDRIA ARE CONTINUOUSLY BREAKING AND …………………………… FOR HOMEOSTASIS. THE ……….. TUBULE WINDS AROUND THE MITOCHONDRIA - IT IS IMPORTANT FOR ………………………. THE COMPONENTS OF FISSION OR …………………… BUT THIS IS NOT TOTALLY UNDERSTOOD

Answer 17

FISSION
REFORMING
ER
RECRUITING
SIGNALLING

Question 18

THERE IS A LOT OF CONTRIBUTION OF THE CYTOSKELETON AS TO THE POSITIONING OF MEMBRANE CONTACT SITES
TRUE OF FALSE

Answer 18

TRUE