L12 Vesicular Traffiking II Flashcards

1
Q

HOW IS INACTIVE GDP CONVERTED TO ACTIVE GTP

A

BY GUANINE NUCLEOTIDE EXCHANGE FACTORS

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2
Q

HOW IS ACTIVE GTP CONVERTED TO INACTIVE GDP

A

BY GTPASE ACTIVATING PROTEINS

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3
Q

WHERE IS GDP USUALLY FOUND

A

IN CYTOSOL

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4
Q

WHERE IS GTP USUALLY FOUND

A

MEMBRANE ASSOCIATED

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5
Q

GIVE AN EXAMPLE OF A GTPASEE

A

RAS

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6
Q

WHAT ARE THE COMPONENTS OF COPII

A

GTPASE: SAR1
ADAPTOR: SEC23/24
COAT: SEC13/31

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7
Q

WHAT IS THE PROCESS OF COPII TRANSLOCATION

A
  1. THE SIGNAL OF THE CARGO WILL ENABLE THE EXIT RECEPTOR TO RECRUIT ADAPTOR PROTEINS
  2. EXIT RECEPTORS LINK THE CARGO WITH THE COPII COAT
  3. IN ORDER TO IDENTIFY THE ACTIVE SITE, SAR1 MUST BE IN ITS ACTIVE FORM
  4. ADAPTOR PROTEINS RECOGNISE AND SELECT CARGO ENSURING SPECIFICITY
  5. THE COAT PROTEINS SEC13/31 FACILITATE THE PINCHING OFF OF THE BUD
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8
Q

WHAT ARE THE MINIMUM REQUIREMENTS FOR COPII FORMATION

A

CYTOSOL (GEF, ADAPTOR PROTEINS AND COAT)
ATP
GTP

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9
Q

HOW WERE THE MINIMUM REQUIREMENTS FOR COPII FORMATION FOUND

A

RECONSTITUTION ASSAY REMOVING MEMBRANE COMPONENTS.
ONLY HAD MEMBRANE PROTEIN CONTAINING RIBOPHORIN, A RESIDENT ER PROTIEN AND P58 A COPII CARGO PROTEIN. FOUND COPII VESICLE COULD BE FORMED ONLY BY ADDING CYTOSOL, ATP AND GTP.
CYTOSOL WAS FURTHER FRACTIONED TO FIND THE IMPORTANT COMPONENT OF GEF, ADAPTOR PROTEINS AND COAT.

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10
Q

WHAT IS THE EFFECT OF MUTATIONS IN GTPASES

A

CONTITUITIVELY ACTIVE

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11
Q

WHAT IS THE EFFECT OF A GDP MUTANT

A

SEQUESTER GEF

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12
Q

WHAT IS THE EFFECT OF A FTP MUTANT

A

CANNOT HYDROLYSE GTP

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13
Q

TRANSPORT THROUGH THE …………………. PATHWAY IS MEDIATED VIA …………………….. VESICLES AND MICROTUBULE ……………………

A

SECRETORY
COATED
TRACKS

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14
Q

WHAT IS CRANIO-LENTICULO-SUTURAL DYSPLASIA

A

AUTOSOMAL RECESSIVE DEFECT IN SEC23A
LARGE DILATIONS OF ER MUTANT CELLS
COPII COATED VESICLE FORMATION AFFCETED BY SEC23A F382L

NOT ALL CARGO IS AFFECTED, SOME CARGOS INVOLVING PROCOLLAGENS ARE MORE AFFECTED.

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15
Q

WHY ARE NOT ALL TISSUES AFFECTED IN CRANIO LENTICULO SUTURAL DYSPLASIA

A

A PARALOGUE OF SEC23A , SEC23B, CAN COMPENSATE

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16
Q

CRANIO LENTICULO SUTURAL DYSPLASIA IN ZEBRA FISH YIELDS WHAT

A

DEFECTS IN SKELETON AND CARTILAGE DEPOSITION

17
Q

WHAT IS THE TRANSPORT PROCESS FOR PROCOLLAGEN

A

IT IS KNOWN THAT COPII IS REQUIRED FOR SECRETION BY HOW IS UNKNOWN DUE TO THE SIZE OF PROCOLLAGEN