L11 Flashcards

1
Q

The periodontium: “Around the tooth” - The support system for the tooth

A

Cementum
Alveolar bone
Periodontal ligament (PDL)
Gingiva: Tooth-associated and gingival ligaments

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2
Q

Hertwig’s epithelial root sheath (HERS)

what is it?

A

Extension of enamel organ, transforms from cervical loop

Bilayer structure of IEE and OEE

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3
Q

Hertwig’s ERS

What does it do?

A

“Architect” of the root defining size and shape (morphogenesis)
Induces root odontoblast differentiation

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4
Q

But how do you get multiple roots?

A

Epithelial growth – epithelial interradicular process(es) = tongues of epithelium growing toward one another

Apical foramina (plural

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5
Q

Hertwig’s epithelial root sheath (HERS):

A

Differentiation of the root
Differentiation of root odontoblasts
Epithelial-mesenchymal signaling

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6
Q

Dental papilla:

A

Undifferentiated ectomesenchymal cells

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7
Q

HERS induces dental papilla cells to

A

differentiate to pre-odontoblasts, then odontoblasts

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8
Q

Root dentin forms in step with

A

HERS proliferation

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9
Q

HERS is a

A

transient structure

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10
Q

HERS disintegrates shortly after

A

inducing root odontoblast differentiation

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11
Q

Some HERS cells become

A

epithelial rests of Malassez (ERM)

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12
Q

Epithelial rests of Malassez (ERM)

A

ERM appear as clumps, strands, or networks of cells in the PDL
Surrounded by basement membrane
Sometimes close to root, sometimes several cell layers away
Function(s)? Can cause cysts. Also speculated to be involved with periodontal homeostasis or regeneration, but not proven…

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13
Q

Root dentin

continuous with

A

crown dentin

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14
Q

Dentinogenesis largely the same as crown

A

(exception: Interacting with IEE of HERS instead of IEE of enamel organ)

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15
Q

Cells:

A

Ectomesenchyme&raquo_space; Dental papilla&raquo_space; Pre-odontoblasts&raquo_space; Odontoblasts
Types: Mantle dentin, circumpulpal dentin, predentin, primary/secondary/tertiary dentin, etc.

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16
Q

Epithelial-mesenchymal signaling in root

HERS

A
Smad4 transcription factor (TF)
Sonic hedgehog (SHH) secreted signal → papilla cells
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17
Q

Dental papilla

A

Induces Gli1 TF
Downstream Nfic TF
Odontoblast differentiation

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18
Q

Without Nfic, dental papilla cannot respond to

A

HERS signaling and cells do not differentiate into odontoblasts
Result: Rootless teeth!

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19
Q

Developmental root defects

A

Defects of HERS growth and/or root dentin formation
Can make teeth prone to breakage, exfoliation, ankylosis, or cause difficult extraction and other issues

Dilaceration: deformity in shape/direction
“Rootless teeth”
Taurodontism: large pulp chamber at expense of root/furcation

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20
Q

Cementum comes in two main types

Defined by:

A

Presence/absence of cells within its matrix
Origin of collagen fibers of the matrix

Major types you need to be familiar with:
Acellular cementum = Acellular extrinsic fiber cementum (AEFC) = Primary cementum
Cellular cementum = Cellular intrinsic fiber cementum (CIFC) = Secondary cementum

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21
Q

Acellular cementum =

A

Acellular extrinsic fiber cementum (AEFC) = Primary cementum

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22
Q

Cellular cementum =

A

Cellular intrinsic fiber cementum (CIFC) = Secondary cementum

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23
Q

Cellular mixed stratified cementum (CMSC): A mix of =

A

alternating acellular and cellular layers

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24
Q

2 major types of cementum:

A

primary accellular cementum (covers 2/3rds of root).

Secondary Acellular cementum (covers apical 1/3rd of root)

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25
Acellular afibrillar cementum
at CEJ
26
Acellular cementum
Cervical 2/3 of root Primary cementum = formed first Acellular extrinsic fiber cementum (AEFC) No cells included
27
Cellular cementum
Apical 1/3 of root Secondary cementum = second formed Cellular intrinsic fiber cementum (CIFC) Cementocytes included
28
Cementum: Attachment: Cementum important for
strong periodontal structure; “cementing” the tooth in the socket (acellular cementum primarily)
29
Cementum: Protecting root from
resorption and repairing resorption pits
30
Cementum: Adjusting
tooth position (Cellular cementum only)
31
Cementum: Sealing
dentin tubules – hydrodynamic theory of dental sensitivity, inhibiting bacterial invasion
32
Cementum composition and properties
``` Composition (similar to bone, dentin) ~50% inorganic: Mineral (hydroxyapatite) 35% organic: Collagen type I (90%), other non-collagenous proteins and glycosaminoglycans (10%) 15% water Physiology (different from bone) Avascular Non-innervated No turnover – growth by apposition ```
33
Classic hypothesis for cementum origins:
Ectomesenchyme > dental follicle > cementoblast | Fenestration of HERS allows follicle cells (pre-cementoblasts) to access root surface
34
Alternative hypothesis for cementum origins:
Dental epithelium > HERS > Epithelial-mesenchymal transformation to cementoblast
35
Cementoblasts
Origin: (Thought to be derived) from dental follicle (ectomesenchyme) Function: Make acellular and cellular cementum Products: Collagens, extracellular matrix (ECM) proteins, enzymes that promote cementum mineralization Fate: Remain in PDL close to cementum surface, regulate slow cementum growth throughout life; direct cementum repair
36
Cementocytes
Origin: A subset of cementoblasts becomes embedded in cellular cementum matrix (i.e. from dental follicle, ectomesenchyme) Function: ???????? Products: Much less than cementoblasts Features: Reside in lacuna (small space in the matrix), extend dendrites (cell processes) through canaliculi (small tunnels) to communicate and receive nutrients Fate: Some remain in lacunae for life Some deep lacunae appear empty- cementocyte death?
37
Dental follicle (dental sac)
Ectomesenchymal origin Precursors to: Cementoblasts, PDL fibroblasts, osteoblasts We don’t know exactly how specific follicle cells become specific differentiated cells, but location is part of it
38
Root dentin –
the “scaffold” for cementum formation
39
HERS disintegrates, exposing
root dentin surface
40
Cementoblast differentiation | From
dental follicle | Remain on/near root surface
41
Initial collagen fibers
Cementoblasts secrete these Intermingle with dentin at the CDJ These short fibers are intrinsic, not yet connected with PDL
42
Dentin-cementum junction (DCJ)
Cementum initial collagen fiber bundles intermingle with dentin collagen fibers Dentin completes mineralization DCJ remains a less hard “cushion” interface between cementum and dentin
43
PDL Fibroblasts
Produce primary collagen fiber bundles of PDL space | Stitched to first cementum intrinsic fibers
44
Extrinsic fibers
Continuity of extrinsic fibers and initial intrinsic fibers | These will become mineralized Sharpey’s fibers within cementum
45
Extrinsic fibers are the
MAJOR fiber group for acellular cementum
46
Extrinsic fibers enter
acellular cementum at HIGH DENSITY
47
Extrinsic fibers are critical to the
FUNCTION of acellular cementum
48
Dense, highly organized Sharpey’s fibers (in red) inserting into both the
acellular cementum and alveolar bone | These are mineralized collagen fiber bundles providing strong anchorage of tooth-PDL-bone
49
Additional views of acellular cementum and Sharpey’s fibers
Note lines perpendicular to root surface– mineralized collagen (Sharpey’s) fibers
50
Continuation of cementogenesis
Mineralization of fibers Cementoblasts promote hydroxyapatite deposition between and within collagen fibers Sharpey’s fibers are mineralized collagen fibers continuous with PDL, also found in alveolar bone
51
Collagen substrate (type I and others) -
the scaffold
52
Cells secrete non-collagenous extracellular matrix (ECM) proteins that participate in
mineral precipitation
53
Cells direct mineralization in and between
collagen fibers, e.g. by ECM proteins and enzymes
54
Progressive mineralization of
extrinsic collagen fibers
55
Progressive mineralization of
extrinsic collagen fibers
56
Mechanism of acellular cementum mineralization
``` Fiber fringe (FF) before cementum initiation FF becomes engulfed and mineralized = Sharpey’s fibers Mineralization continues slowly over time (~3 mm/yr) ```
57
(Secondary) Cementoblasts | Produce
cementum matrix rapidly – cementoid | Produce many intrinsic collagen fibers, deposit the cellular cementum ECM
58
Cellular cementum: Often minimal or absent in
incisors and canines = little role in tooth attachment
59
Cellular cementum: “Adaptive cementum” maintains
tooth in proper occlusal position by compensating for enamel attrition throughout life
60
Cellular cementum can repair
cementum resorption anywhere on root
61
Mechanism of cellular cementum mineralization
A clear unmineralized cementoid Equivalent to predentin or osteoid Cementoblast secretes collagen and other proteins >> Time lag >> Matrix mineralizes Conditions that delay/inhibit mineralization may affect cementoid similarly to osteoid
62
Cementocytes | Embedded in
cellular cementum matrix Connected to one another and surface (PDL) Equivalent to osteocytes in bone?
63
Both acellular and cellular cementum continue
growing SLOWLY throughout life
64
No remodeling/turnover– cementum
appositional growth (adding to the existing layer)
65
Longitudinal lines/striations/appositional growth lines indicate
successive layers | BUT resorption does sometimes occur…
66
Reparative cementum
Following root cementum resorption by osteoclasts/odontoclasts Repair cementum fills resorption pit (Howship’s lacuna) Reparative cementum is often cellular, regardless of location (i.e. even on cervical root) Concerns: Is new cementum well bonded with dentin (i.e. quality of CDJ)? Is new cementum well attached to PDL (i.e. density of extrinsic fibers?)
67
What can go wrong with cementum? | too little
Too little: cementum aplasia or hypoplasia
68
What can go wrong with cementum? | too much
Too much: hypercementosis, possibly leading to ankylosis
69
What can go wrong with cementum? Loss of cementum
Loss of cementum: External root resorption
70
Hypophosphatasia
Rare skeletal disease Mutations in ALPL, gene for tissue-nonspecific alkaline phosphatase (TNAP protein) TNAP breaks down pyrophosphate (PPi), an inhibitor of mineralization HPP: High PPi Acellular cementum aplasia or hypoplasia
71
HPP affects
bone (skeletal and craniofacial), dentin, and enamel
72
Acellular cementum most dramatically HPP
affected tissue in terms of severity and prevalence
73
HPP | Defective/absent cementum →
Loose teeth, premature loss of primary and/or secondary teeth
74
Hypercementosis
Excessive cementum growth Trauma, genetic disease, idiopathic Generally asymptomatic, may cause ankylosis and difficulty in extraction Unusual example of genetic hypercementosis that is opposite of HPP
75
What factors are important in mineralization? | acellular cementum
Bone sialoprotein (BSP) First discovered in bone, but also present in dentin and cementum Promotes hydroxyapatite mineral formation Critical role in acellular cementum formation BSP knock-out mouse has cementum hypoplasia, PDL detachment, periodontal breakdown No known human equivalent, though phenotype consistent with some aspects of aggressive periodontitis
76
Root resorption (external)
Odontoclasts (osteoclast-like cells) Very common to have “mild” resorption in 1 or more teeth Excessive orthodontic force (usually apical effect) Related to trauma, severe periodontitis, genetic factors “Idiopathic”
77
Multiple idiopathic cervical root resorption
very aggressive, no treatment
78
Root (surface) caries
Bacteria/plaque initiated Exposed root surface Soft, progressive lesion – distinct from clastic resorption
79
Alveolar bone structure
Alveolar process/alveolar bone forms the socket that holds the tooth Tooth-associated bone Cortical plates (buccal/lingual) Trabecular bone (spongiosa) Alveolar plate is the focus for today’s lecture- the most involved with periodontal function More in lecture 11 by Dr. Sun…
80
Alveolar bone:
The partner of cementum Includes extrinsic collagen fiber bundles, mineralized Sharpey’s fibers (similar to acellular cementum) Primary fibers entering bundle bone are larger in diameter and less dense (vs. cementum) Bundle bone,
81
Bundle bone =
Bundle bone = Bone lining the socket, inner aspect facing tooth root
82
Lamina dura=
Radiographic feature of alveolar bone Radiopaque layer lining the socket Increased radiopacity from thick bone without trabeculation, NOT because of increased mineral content
83
Lamina dura is
Lamina dura is opaque (radiopaque)
84
PDL is
radiolucent
85
Cribriform plate = structure pierced by
many small holes
86
Bone remodeling in
response to function – osteoclasts and osteoblasts work in tandem
87
“Clasts” and their role in resorption
Normal remodeling of alveolar bone- FASTEST remodeling bone in the body Normal bone resorption allowing tooth eruption Normal tooth resorption when deciduous teeth are exfoliated Abnormal when clasts resorb the roots of permanent teeth
88
Alveolar bone – tooth interactions | Alveolar bone distributes
occlusal loads
89
Existence of alveolar bone depends on
this interaction with dentition
90
“Mechanostat” theory of bone loading
Bone loading causes growth (e.g. tennis player effect) Bone unloading causes loss (e.g. astronaut effect) Edentulous mandible gradually loses alveolar bone
91
The periodontal ligament (PDL)
Soft fibrous connective tissue between tooth and alveolar bone (i.e. occupies the periodontal space)
92
Ligament =
Fibrous connective tissue connecting bone to bone
93
Periodontal ligament connects bone to tooth in a unique joint called a
gomphosis, or tooth socket | Normal width ~ 0.1 - 0.4 mm (BUT varies by person and tooth) is tightly regulated in health
94
Functions of PDL | Supportive:
Primary collagen fibers attach tooth to bone
95
Functions of PDL | Nutritive:
Blood supply to cells of the region, including cementoblasts and cementocytes
96
Functions of PDL | Sensory:
Innervated for sensing position and pain
97
Functions of PDL | Defensive:
Delivers immune cells including macrophages and neutrophils
98
Functions of PDL | Maintenance/Reparative:
Contains stem and progenitor cells that can repair or regenerate PDL, bone, cementum
99
Functions of PDL | Adaptive:
Based on mechanical loading (functional input), adapts fiber orientations and influences neighboring alveolar bone remodeling Optimal arrangement to accept and distribute tensile forces from mastication Even after orthodontic tooth movement, PDL returns to same width
100
Composition of PDL | Ground substance”
Amorphous background material | Proteins, proteoglycans, water
101
Composition of PDL | Collagen fibers
Collagen types I, III, XII major types Fiber bundles – “spliced rope” 97% of fibers
102
Composition of PDL | Oxytalan fibers
Small elastic fibers, support collagen fibers and blood vessel walls 3% of fibers NO elastic fiber bundles = PDL is more stiff for withstanding forces
103
PDL principal fiber groups Collagen fiber bundles spanning PDL from mature tooth to bone (*except transseptal group is tooth-tooth) Function:
Resist intrusive and extrusive forces, tipping and lateral movements Defined by location or orientation 6 groups: TAHOAI Oblique group is predominant- main group resisting occlusal loads (intrusive force)
104
Transseptal fibers travel from
tooth to tooth
105
Fibroblasts
Majority of cells in PDL Secrete and remodel matrix Intimately associated with collagen fibers- cells may act as mechanotransducers
106
Other PDL cells
``` Cementoblasts Osteoblasts, Osteoclasts Epithelial rests of Malassez (ERM) Stem/progenitor cells: Ability to differentiate and regenerate Immune cells ```
107
PDL is
well vascularized Superior and inferior alveolar arteries Perforating vessels, sometimes called alveolar or intra-alveolar – MAJOR route Apical routes and gingival vessel routes also
108
Venous drainage in - PDL
axial direction
109
Gingival crevicular fluid (GCF)
Found in the sulcus/gingival margin Transudate from vasculature Diagnostic value
110
(1) Free endings, tree-like
Most common, found along the length of the root | Nociceptors and mechanoreceptors
111
(2) Ruffini endings
(slowly adapting, found in skin) Found near apex Mechanoreceptors
112
(3) Coiled endings
Mid-region of PDL | Function unknown
113
(4) Encapsulated endings
Found near apex | Function unknown
114
Cementicle
Ectopic cementum in PDL, attached or unattached
115
Ankylosis
Cementum-bone fusion | Loss of PDL space
116
Gingival epithelium
Oral epithelium Sulcus epithelium Junctional epithelium
117
Junctional epithelium
Enamel and cementum Barrier to microbial invasion Fast turnover (Recall, this is derived from leftover enamel organ/reduced enamel epithelium)
118
Gingival ligament groups
Not PDL, but collagen fiber bundles spanning from tooth/bone to gingiva/connective tissue In lamina propria (underlying connective tissue) of the gingiva Function: Resist gingival displacement 4 groups: CDDA (transseptal group sometimes included, but we are grouping with PDL)