L1 T cell activation

Question 1

5 stages of T cell activation

Answer 1

1. presentation & recognition of antigen
2. Activation (early phase) initiation of signal transduction cascade MEMBRANE
3. Activation (intermediate) signal transduction into CELL
4. Activation (late phase) initiation gene transcription/ nuclear events
5. functional response effector cell end-action

Question 2

What do B & Tc require to read in order to be activated

Answer 2

B cell - must see entire structure (can only be activated by protein in normal tertiary structure)
Tc - only sees sequence (denaturing protein has no effect)

Question 3

To allow Tc proliferation what must happen

Answer 3

antigen must be processed: ‘digested.’ Either processed before the cell is fixed or fix cell and add preprocessed antigen

Question 4

Describe Cytosolic presentation

Answer 4

For MHC-I (CD8) used to process endogenous antigens

Question 5

Describe Endocytic presentation

Answer 5

For MHC-II (CD4) - exogenous proteins (protein taken from outside into cell via endocytosis

Question 6

True or false: the Tc that process your proteins are deleted at birth

Answer 6

True: prevents autoimmunity

Question 7

MHC structure - what is the peptide binding cleft for CD8?

binding cleft for CD4?

Answer 7

1. CD8 attaches to a3 domain

2. CD4 attaches to B2 domain