L 15 March 13 Fayyad Presentation

Question 1

Define organotropic metastasis

Answer 1

NA
- Seed and soil hypothesis:
Metastatic tumor cells (seed) + organ microenvironment (soil) -> organotropic metastasis

Question 2

Define exosomes

Answer 2

Small extracellular membrane vesicles with fxnal biomolecules

Question 3

How do exosomes induce pre-metastatic niche formation?

Answer 3

Induces hallmarks of pre-metastatic niche formation by inducing vascular leakiness, inflammation and bone marrow progenitor cell recruitment

Question 4

What 3 things cause pre metastatic niche formation?

Answer 4

1. Hematopoietic bone marrow cells expressing VEGFR
2. Stromal cells from primary tumor
3. Exosomes from primary tumor

Question 5

Are exosomes responsible for organotropic metastasis?

Answer 5

NA

Question 6

Which compartment of exosomes are responsible for organometastasis?

Answer 6

NA

Question 7

Can metastasis be preventing by blocking such componenets?

Answer 7

NA

Question 8

Can such components be used as a biomarker to predict future metastatic sites?

Answer 8

NA

Question 9

What are the 3 exosomes studied in this exp?

Answer 9

1) 4175-LuT exosomes: From cell line that colonizes lung after metastasis.
2) 1833-BoT exosomes: From cell line that colonizes Bone.
3) 831-BrT exosomes: From cell line that colonizes Brain

Question 10

How did exosomes prepare a pre-metastatic niche?

Answer 10

FMI-43 dye labelled
4175-LuT exosomes were
injected into mice
->
3 days
->
Electron Microscopy of
lung sections
->
Exogenous exosomes can be detected in lung sections along with endogenous organotropic
factors forming a pre-metastatic niche

Question 11

How did exosomes redirect metastatic distribution?

Answer 11

NA