Krebs Cycle

Question 1

Location of kreb cycle

Answer 1

Mitochondria, inner membrane (cristae) + matrix

Question 2

production of acetyl CoA

Answer 2

• Pyruvate + CoA + NAD -> acetyl CoA + NADH + C02

- irreversible step

Question 3

catalyst of Acetyl CoA production

Answer 3

pyruvate dehydrogenase

Question 4

Features of pyruvate dehydrogenase

Answer 4

• 3 enzymes + 5 coenzymes

- inhibition -> acetyl CoA + NADH (feed back)

Question 5

Acetyl CoA + oxaloacetate = + enzyme

Answer 5

Citrate

• Citrate synthase
• Co A regenerated

Question 6

Citrate ->

Answer 6

Isocitrate

- aconitase

Question 7

Isocitrate ->

Answer 7

• alpha ketoglutarate
• Isocitrate dehydrogenase
• C02, NADH

Question 8

Alpha ketoglutarate ->

Answer 8

• succinyl CoA
• alpha ketoglutarate dehydrogenase
• CO2 + NADH

Question 9

Succinyl CoA ->

Answer 9

• > succinate
• Succinyl CoA
• GTP

Question 10

Succinate ->

Answer 10

• Fumarate
  Succinate dehydrogenase
• only enzyme not in matrix - on membrane
  FADH2

Question 11

Fumarate ->

Answer 11

• Malate

Fumarase

Question 12

Malate ->

Answer 12

• oxaloacetate
  malate dehydrogenase
• NADH

Question 13

Total products

Answer 13

3 CO2 (1 from acetyl CoA)
4 NADH (1 from acetyl CoA)
1 FADH2
1 GTP

Question 14

3 main control points

Answer 14

• citrate synthase
• isocitrate dehydrogenase
• alpha ketoglutarate

Question 15

Inhibition Of Citrate synthase

Answer 15

• Citrate

- ATP

Question 16

Inhibition of alpha ketoglutarate dehydrogenase

Answer 16

• ATP

- NADH

Question 17

Activation of alpha ketoglutarate

Answer 17

• ADP

Question 18

Activation of isocitrate

Answer 18

• ADP

Question 19

Effect of high Succinyl CoA

Answer 19

• acts as an inhibitor for whole cycle

Question 20

Pyruvate Dehydrogenase Complex deficiency features

Answer 20

X linked
Persistent Acidosis
|Neurological development impacted