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CLIN CHEM > KLUBSY: LABORATORY SAFETY > Flashcards

KLUBSY: LABORATORY SAFETY Flashcards

1
Q

Used to remove particles that may be harmful to the employee who is working with potentially infectious biologic specimens

A

Biosafety cabinets (BSCs)

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2
Q

This must be used when performing procedures that generate
infectious aerosols

A

BSC

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3
Q

For BSC Class I, what is its face velocity?

A

75

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4
Q

for BSC Class II, A1, what is its face velocity?

A

75

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5
Q

For BSC Class II, A2, what is its face velocity?

A

100

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6
Q

For BSC Class II, B2, what is its face velocity?

A

100

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7
Q

For BSC Class II B2, what is its face velocity?

A

100

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8
Q

In at front
through HЕРА
to the outside
or into the
room through
HEPA. What BSC Class is this?

A

BSC Class I

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9
Q

What is the airflow pattern for BSC Class II, A1?

A

R - 70%
E - 30%

*R for recirculated and E for exhausted

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10
Q

What is the airflow pattern for BSC Class II, A2?

A

R = 70%
E = 30%

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11
Q

What is the airflow pattern for BSC Class II, B1?

A

R = 30%
E = 70%

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12
Q

What is the airflow pattern of BSC Class II, B2?

A

No recirculation,
total exhaust
to outside. Therefore,

R = 0%
E = 100%

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13
Q

This BSC Class only exhausted air is filtered by HEPA filter

A

Class I

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14
Q

In this BSC Class, air entering and air exhausted is filtered

A

Class II

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15
Q

In this BSC Class, gloves are attached

A

Class III

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16
Q

These are required to contain and expel noxious and hazardous
fumes from chemical reagents

A

chemical fume hoods

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17
Q

used when working with some highly toxic and volatile
chemical

A

chemical fume hoods

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18
Q

a partially enclosed ventilated work space for volatile
chemicals

A

chemical fume hoods

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19
Q

T/F: Chemical fume hoods are used for storage of hazardous chemicals

A

FALSE.

NOT used

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20
Q

Chemical fume hoods Periodically evaluate what?

A

Periodically evaluate ventilation

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21
Q

Chemical fume hoods periodically evaluate ventilation by what?

A

by measuring face velocity

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22
Q

Chemical fume hoods periodically evaluate ventilation by measuring face velocity using a what?

A

with a calibrated velocity meter

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23
Q

Specialized clothing or equipment worn by an employee
for protection

A

Personnel protective equipment (PPE)

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24
Q

These PPE protect the hands and arms
when using caustic chemicals.

A

gloves and rubberized sleeves

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25
Q

Equipment for Face and Eye protection?

A

goggles and face shield

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26
Q

What does HEPA mean?

A

High-efficiency particulate air

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27
Q

What is donning?

A

putting ON of PPE

*dONning

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28
Q

what is doffing?

A

Putting off of PPE

*dOFFing

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29
Q

What is the correct sequence of donning PPE?

A

Gown > masks/respirator > goggles/face shield > gloves

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30
Q

What is the correct sequence for doffing PPE?

A

Gloves > goggles/face shield > gown > mask/respirator > perform HANDWASHING

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31
Q

Single most important method to reduce infection
transmission

A

Handwashing

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32
Q

what must be utilized in proper handwashing?

A

Use soap and warm water

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33
Q

scrubbing time for handwashing?

A

20 seconds

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34
Q

what is an IMPORTANT step in handwashing?

A

mechanical friction

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35
Q

4 types of BIOLOGIC SAFETY

A

1-universal precaution
2-body substance isolation
3- standard precautions
4-transmission based precautions

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36
Q

Apply to blood and to other body fluids containing visible
blood.

A

Universal precaution

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37
Q

Recommend the use of PPE, handwashing after removal
of gloves, for handling and disposal of needles and other
sharps

A

Universal precaution

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38
Q

Emphasized the avoidance of contact with potentially
infectious, moist body fluid (except sweat) regardless of
the presence or absence of blood

A

Body substance isolation

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39
Q

Handwashing after glove removal not required unless
hands visibly soiled

A

Body substance isolation

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40
Q

Applies to all body fluids, secretions, excretions, (except sweat) and tissue specimens

A

standard precautions

41
Q

Applies to human-based standards or calibrators, quality
control materials, and proficiency testing materials

A

standard precautions

42
Q

Applies to non-intact skin and mucous membranes

A

standard precautions

43
Q

what are the 3 categories of transmission based precautions?

A

-airborne
-droplet
-contact

44
Q

this biological safety type has 3 categories: airborne, droplet, contact

A

transmission-based precautions

45
Q

Used when SP (standard precaution) alone are insufficient

A

transmission based precautions

46
Q

Used when for patients with known or suspected infection

A

transmission-based precautions

47
Q

Required by OSHA to minimize the risk of exposure to
bloodborne pathogens

A

exposure control plan

48
Q

the exposure control plan is required by?

A

OSHA (Occupational Safety and Health Administration)

49
Q

Ensures protective safety of laboratory staff to potential
infectious bloodborne pathogen

A

Exposure control plan

50
Q

Process that eliminates many or all pathogenic
microorganisms, except bacterial spores, on inanimate
objects

A

disinfection

51
Q

Widely used disinfectant?

A

household bleach or sodium
hypochlorite

52
Q

What is the ratio to make sodium hypochlorite disinfectant?

A

1 part bleach : 9 parts H2O

53
Q

What are the 8 steps in case of a spill?

A

1-Alert others in area of the spill.
2-Wear appropriate protective equipment.
3-Use mechanical devices to pick up broken glass or other
sharp objects
4- Absorb the spill with paper towels, gauze pads, or tissue
5-Clean the spill site using a common aqueous detergent.
6-Disinfect the spill site using approved disinfectant or 10%
bleach, using appropriate contact time.
7-Rinse the spill with water
8-Dispose of all materials in appropriate biohazard
containers

*accdg to Turgeon, the 1st step in case of a spill is step #4.

54
Q

A plan that provides safety policies, procedures & work
practices for all hazardous chemical

A

chemical hygiene plan

55
Q

regulating and reducing exposure of lab personnel to
hazardous chemicals

A

chemical hygiene plan

56
Q

this personnel must be designated for any lab
using hazardous chemicals

A

chemical hygiene officer

57
Q

Informs a worker about the hazards of chemicals in the
workplace under normal conditions of use and
foreseeable emergencies

A

hazard communication standard

58
Q

What does SDS mean?

A

safety data sheet

59
Q

Requires that the chemical manufacturer, distributor,
or importer provides Safety Data Sheet (SDS) for each
HAZARDOUS chemical

A

hazard communication standard

60
Q

what is the gold standard for chemical safety?

A

hazard communication standard

61
Q

Recall:

which of the following is true for SDS?

62
Q

what are the 6 information provided on every label of all chemicals?

A

1-product identifier
2-signal word
3-hazard statements
4-pictogram
5-precautionary statement
6-name, address, and phone number of supplier

63
Q

GHS (Globally harmonized system Pictograms and Hazards:

Carcinogen

A

Health hazard

64
Q

GHS (Globally harmonized system Pictograms and Hazards:

mutagenicity

A

health hazard

65
Q

GHS (Globally harmonized system Pictograms and Hazards:

reproductive toxicity

A

health hazard

66
Q

GHS (Globally harmonized system Pictograms and Hazards:

target organ toxicity

A

health hazard

67
Q

GHS (Globally harmonized system Pictograms and Hazards:

aspiration toxicity

A

health hazard

68
Q

GHS (Globally harmonized system Pictograms and Hazards:

flammable

69
Q

GHS (Globally harmonized system Pictograms and Hazards:

pyrophorics

70
Q

GHS (Globally harmonized system Pictograms and Hazards:

self-heating

71
Q

GHS (Globally harmonized system Pictograms and Hazards:

emits flammable gas

72
Q

GHS (Globally harmonized system Pictograms and Hazards:

self-reactives

73
Q

GHS (Globally harmonized system Pictograms and Hazards:

organic peroxides

74
Q

GHS (Globally harmonized system Pictograms and Hazards:

irritant (skin and eye)

A

exclamation mark

75
Q

GHS (Globally harmonized system Pictograms and Hazards:

skin sensitizer

A

exlamation mark

76
Q

acute toxicity (HARMFUL)

A

exclamation mark

77
Q

GHS (Globally harmonized system Pictograms and Hazards:

narotic effects

A

exclamation mark

78
Q

GHS (Globally harmonized system Pictograms and Hazards:

respiratory tract irritant

A

exclamation mark

79
Q

GHS (Globally harmonized system Pictograms and Hazards:

hazardous to ozone layer (non-mandatory)

A

exclamation point

80
Q

GHS (Globally harmonized system Pictograms and Hazards:

gases under pressure

A

gas cylinder

81
Q

GHS (Globally harmonized system Pictograms and Hazards:

skin corrosion/burns

82
Q

GHS (Globally harmonized system Pictograms and Hazards:

eye damage

83
Q

GHS (Globally harmonized system Pictograms and Hazards:

corrosive to metals

84
Q

GHS (Globally harmonized system Pictograms and Hazards:

explosives

A

exploding bomb

85
Q

GHS (Globally harmonized system Pictograms and Hazards:

self reactives

A

exploding bomb

86
Q

GHS (Globally harmonized system Pictograms and Hazards:

organic peroxides

A

exploding bomb

87
Q

GHS (Globally harmonized system Pictograms and Hazards:

oxidizers

A

flame over circle

88
Q

GHS (Globally harmonized system Pictograms and Hazards:

aquatic toxicity

A

environment (non mandatory)

89
Q

GHS (Globally harmonized system Pictograms and Hazards:

acute toxicity (fatal/toxic)

A

Skull and crossbones

90
Q

Important part of the GHS and global chemical safety

A

Safety Data Sheet

91
Q

includes information such as the properties of each
chemical; the physical, health, and environmental health
hazards; protective measures; and safety precautions
for handling, storing, and transporting the chemical.

92
Q

Must be provided in a user-friendly, specific 16-section
format

93
Q

How many sections are there in SDS?

94
Q

What are the 16 sections of SDS?

A

Identification

Hazard Identification

Composition/Information on Ingredients

First-aid Measures

Firefighting Measures

Accidental Release Measures

Handling and Storage

Exposure Controls/Personal Protection

Physical and Chemical Properties

Stability and Reactivity

Toxicological Information

Ecological Information

Disposal Considerations (Non-mandatory)

Transport Information

Regulatory Information

Other Information

95
Q

Among the most hazardous materials iin the clin chem lab

A

flammable/combustible chemicals

96
Q

Classified according to flash point

A

flammable/combustible chemicals

97
Q

flammable/combustible chemicals are classified accdg to what?

A

Flash point

CLIN CHEM (5 decks)

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