Key Area 1.2- Replictaion of DNA

Question 1

Strand of nucleic acid running from a sugar to a phosphate

Answer 1

3’-5’

Question 2

Enzyme that adds free complementary DNA nucleotides during replication of DNA

Answer 2

DNA polymerase

Question 3

Enzyme from hot-spring bacteria, used in PCR

Answer 3

Heat-tolerant DNA polymerase

Question 4

DNA strand that is replicated in fragments

Answer 4

Lagging strand

Question 5

DNA strand that is replicated continuously

Answer 5

Leading strand

Question 6

Enzyme that joins DNA fragments to make the lagging strand

Answer 6

Ligase

Question 7

Part of experimental design in which a negative result is expected; in PCR, a tube with no DNA to be amplified

Answer 7

Negative control

Question 8

Method of amplifying sequences of DNA in vitro

Answer 8

Polymerase chain reaction(PCR)

Question 9

Part of experimental design in which a positive result is expected; in PCR, a tube with DNA which is already known to be amplified by the method being used

Answer 9

Positive control

Question 10

Short complementary strand of DNA

Answer 10

Primer

Question 11

Formation of identical copies of DNA molecules

Answer 11

Replication

Question 12

DNA strand in which a complementary copy is made

Answer 12

Template strand