Karyotyping quiz

Question 1

• sex chromosomes content
• presence or absence of individual chromosomes
• nature or extent of chromosomal aberrations

Answer 1

KARYOTYPE

Question 2

samples can be used for Karyotyping?

Answer 2

Samples can be taken from:
* blood
* bone marrow
* skin sample from an adult
* amniotic fluid
* extra embryonic cells from chronic villi from an unborn child

These samples allow for chromosomal analysis.

Question 3

stimulate mitosis.

Answer 3

Lectins

Question 4

Cell division is arrested by using:

Answer 4

Colchicine

Question 5

How can chromosomes be distinguished?

Answer 5

Chromosomes can be distinguished by:
* length of the arms
* shape
* general appearance

Question 6

Term used to describe a cell with 3 sets of chromosomes(3n)

Answer 6

Triploidy

Question 7

XXY sex chromosme pertains to:

Answer 7

Klinefelter’s Syndrome

Question 8

A female with “single X” chromosome instead of “double X” sex chromosome result into

Answer 8

Turner Syndrome

Question 9

*1/1000
*above average in height
*anti social behavior

Answer 9

Jacob Syndrome

Question 10

• dark regions tend to be heterochromatic, late replicating, and AT rich
• light regions tend to be euchromatic, early-replicating, and GC rich

Answer 10

G-Banding

Question 11

cells are placed that causes them to take on water, thereby increasing their overall size.

Answer 11

Hypotonic Solution

Question 12

chromosome number that is a multiple of the normal haploid set.

Answer 12

Polyploidy

Question 13

It is the condition in which the chromosome number in the cell is not that typical number for the species

Answer 13

Aneuploidy

Question 14

having only one member of a homologous pair.

Answer 14

Monosomy

Question 15

having three copies of a single chromosome.

Answer 15

Trisomy

Question 16

rarely seen in spontaneous abortion and live births

Answer 16

Autosomal Monosomy

Question 17

• most lethal
• accounts for 50% of cases of chromosomal abnormalities causing fetal death
• risk increases after age 35

Answer 17

Autosomal Trisomy?

Question 18

*also known as Patau Syndrome (47,+13).
* occurs in 1/5,000
* survival time at least 1 month

Answer 18

Trisomy 13

Question 19

*also known as Edward Syndrome (47,+18).
* occurs in 1/10,000
* survival time: 2-4 months

Answer 19

Trisomy 18

Question 20

*Down Syndrome (47,+21).
* occurs in 1/700
* 40% have congenital heart defects

Answer 20

Trisomy 21

Question 21

utilizes specific gene locus probes in analyzation of chromosomes

Answer 21

FISH

Question 22

reagent is used in silver staining technique to visualize the nucleolar organization of chromosomes

Answer 22

Silver nitrate

Question 23

The arresting phase of mitosis for karyotyping analysis

Answer 23

Metaphase

Question 24

FISH involves Characterization of

Answer 24

Specific segments of genes

Question 25

Observed in karyotyping

Answer 25

*General appearance of chromosome
*Lenght of chromosomes arms
*Shape of chromosome

Question 26

Trisomy of chromosome 21 results into

Answer 26

Down syndrome

Question 27

is a fluorescent pattern
obtained using quinacrine for staining. The
pattern of bands is very similar to that
seen in G-banding.

Answer 27

Q-banding

Question 28

For karyo typing analysis of a fetus, which of the following is the least ideal sample

*Maternal blood
*Extra-embryonic cells
*Amniotic fluid
*Chorionic villi sample

Answer 28

Amniotic fluid

Question 29

Dye used in karyotyping

Answer 29

Giemsa

Question 30

Its is a technique used to quantify the DNA copy number on a genomic scale

Answer 30

Digital karyotyping