Jham Special Stains and Markers

Question 1

α1 antichymotrypsin

Answer 1

Histiocytes

Question 2

α subunit of S-100

Answer 2

Melanoma and neural tumors

Question 3

AE1/AE3

Answer 3

LOW molecular weight keratin

Question 4

Actin

Answer 4

smooth muscle (oh good, its a leio)

Question 5

Stains Acidic mucopolysaccharides blue

Answer 5

Alcian blue

Question 6

Bcl-1

Answer 6

Mantle cell lymphoma (along with Cyclin-D1)

Question 7

Bcl-2

Answer 7

T and B-cells, follicular lymphoma, SFT

Question 8

Ber-EP4

Answer 8

+ in BCC, - in ameloblastoma

Question 9

What pigment is affected by bleach?

Answer 9

melanin. Hemosiderin remains

Question 10

What in the world is a Bodian stain?

Answer 10

Stain for axons (neurofibroma) - axons stain magenta in a neuroma and bundles stain in a NF

Question 11

Brown and Brenn

Answer 11

microorganisms: actinomycosis

Question 12

Basement membrane of lupus

Answer 12

C3

Question 13

Calponin

Answer 13

smooth muscle specific proteins ( good for myoepithelial cells)

Question 14

CAM5.2

Answer 14

LOW molecular weight keratin

Question 15

C-ANCA - what is it now known as?

Answer 15

cytoplasmic anti-neutrophil cytoplasmic antibodies

Indirect immunofluorescence test for Wegner Granulomatosis, now known as Granulomatosis with Polyangiitis

now known as: PR3-ANCA for proteinase-3

Should also order ELISA for PR3

Question 16

P-ANCA - What is it know known as?

Answer 16

Perinuclear anti-neutrophil cytoplasmic antibodies

Now known as MPO-ANCA (myeloperoxidase)

Question 17

CDX2

Answer 17

intestinal epithelium (intestinal adenocarcinoma)

Question 18

CD1a

Answer 18

LCH

Question 19

CD3

Answer 19

T-cell specific (+ in ALCL)

Question 20

CD4 (two positive conditions)

Answer 20

mycosis fungoides, Angioummunoblastic T-cell lymphoma

Question 21

CD5

Answer 21

T-cells, mantle cells, + in CLL/SLL with CD23, + in Mantle cells lymphoma, negative in MALT lymphoma

Question 22

CD7

Answer 22

T-cells

Question 23

CD10 (aka?)

Answer 23

Aka: CALLA

T-cells, follicular center B-cells (+ in follicular lymphoma, -ve in mantle cells) + in Burkitt, + in RCC

Question 24

CD11c

Answer 24

hairy cell leukemia

Question 25

CD13

Answer 25

macrophage, monocyte lineage

Question 26

CD15/30

Answer 26

Hodgkin Lymphoma (Reed-Sternberg cell)

Question 27

CD20 (aka, what it stains, 2 positive conditions)

Answer 27

aka Leu-26 Pan B-cell marker (not plasma cells) "popcorn" cell in Hodgkin Lymphoma (non-classical RS cell negative for CD15 and CD30, positive for CD19, 20, 22, 79a, Bcl-6 + in MALT lymphoma

Question 28

CD25

Answer 28

Hairy cell leukemia

Question 29

CD30

Answer 29

RS cell, activated T and B cells, + ALCL

Question 30

CD31

Answer 30

Endothelial cells, better for angiosarc than CD34

Question 31

CD34 (cells stained, and 4 relevant entities that stain positive or negative)

Answer 31

endothelial cells / vascular marker Positive: SFT (aka: hemangiopericytoma), DFSP, Spindle cell lipoma Negative: BFH

Question 32

CD45 (aka, cell types, positive/negative conditions)

Answer 32

aka: Leukocyte Common Antigen (LCA) All leukocytes except plasma cells Positive in leukemias, lymphomas Negative: RS cells in Hodgkin

Question 33

CD56 (2 conditions)

Answer 33

Pos: NK T-cell lymphoma (hole in palate) some cells of MNETI

Question 34

CD68 (3 cell types, one positive tumor)

Answer 34

macrophages, histiocytes, osteoclast-like giant cells pos: GCT

Question 35

CD70

Answer 35

RS cell in Hodgkin lymphoma

Question 36

CD79a

Answer 36

B-cells (including plasma cells) Positive in Mantle cell, Follicular, and plasmacytoma

Question 37

CD99

Answer 37

Ewing sarcoma, synovial sarcoma, SFT, mesenchymal chondrosarcoma

Question 38

CD117 (aka, 3 conditions its associated w/)

Answer 38

aka c-kit positive: GIST Adenoid cystic Ca Negative: PLGA

Question 39

CD138

Answer 39

Plasma cells (plasmablastic lymphoma, plasmacytoma)

Question 40

Mum1

Answer 40

Plasma cells (plasmablastic lymphoma, plasmacytoma)

Question 41

CD163

Answer 41

Monocyte / macrophage / histiocyte lineage Positive in BFH, Xanthoma of bone Negative in DFSP

Question 42

CD207

Answer 42

aka Langrin...LCH

Question 43

Which is more sensitive and specific: chromogranin or synaptophysin?

Answer 43

synapto (chromo used as a supplement)

Question 44

CK7

Answer 44

Sinonasal or lung mets (CK7+/CK20-) Positive in synovial sarcoma (with AE1/AE3, CK1)

Question 45

CK14

Answer 45

oncocytic tumors

Question 46

CK20

Answer 46

merkel cell carcinoma, colon cancer, checking for mets (CK7-/CK20+)

Question 47

Congo Red

Answer 47

Amyloid (apple green birefringence on polarized light)

Question 48

Crystal violet

Answer 48

amyloid (metachromatic)

Question 49

Cyclin D-1

Answer 49

Mantle cell lymphoma

Question 50

Desmin

Answer 50

mesenchymal cells: skeletal and smooth muscle, negative in myofibromatosis

Question 51

Factor VIII

Answer 51

endothelial cell stain

Question 52

Factor XIIIa (2 pos, 1 neg)

Answer 52

Positive BFH DF Negative DFSP