jan 17

Question 1

who are larry frye and michael edidin

Answer 1

fusion of human and mouse cells
- tagged proteins in each cell with fluorescent tags (red = humans, green = mouse)

Question 2

technique for studying membrane protein mobility

Answer 2

FRAP - fluorescence recovery after photobleaching

Question 3

protein a is restricted by

Answer 3

random diffusion

Question 4

protein b is restricted by

Answer 4

issues w underlying membrane skeleton

Question 5

protein c is restricted by

Answer 5

being moved in particular direction bc interaction w motor protein

Question 6

protein d is restricted by

Answer 6

other integral proteins

Question 7

protein e is restricted by

Answer 7

fences

Question 8

protein f is restricted by

Answer 8

extracellular materials

Question 9

n linked carbohydrate means it is

Answer 9

aspirigine

Question 10

o linked carbohydrates means it is

Answer 10

serine or threonine

Question 11

what bonds hold peripheral membrane proteins to the membrane - what types of molecules

Answer 11

hydrogen bonds and electrostatic - weak bonds
to lipids or other proteins

Question 12

facts about integral proteins

Answer 12

amphipathic
diffuse across the cytosol
they can make channel functions

Question 13

peripheral membrane proteins usually spend their time at the membrane

Answer 13

false - weak bonds allow them to move a lot

Question 14

membrane proteins are diffused asymmetrically

Answer 14

true - they’re either on one leaflet or the other

Question 15

gpi or glycosyl phosphatidyl inositol faces …

Answer 15

away from cytoplasm

Question 16

4 features of lipid rafts

Answer 16

1. tightly packed
2. sphingolipids (no glycerol - long fatty acid tails)
3. cholesterol
4. receptor proteins binding external chemical signals can move to rafts

Question 17

glycolysis occurs where

Answer 17

in the cytoplasm