IV. Osmoregulation

Question 1

whats a hypertonic solution?

Answer 1

Solution has a higher concentration of solutes than the cell. Water moves out of the cell, causing it to shrink or undergo plasmolysis.

Question 2

whats a hypotonic solution?

Answer 2

Solution has a lower concentration of solutes than the cell. Water moves into the cell, causing it to swell or become turgid.

Question 3

Methodology of observing osmosis in Rhoeo discolor cells?

Answer 3

1. Slice lower epidermis of and place it on a glass slide with water.
2. Cover with a cover slip and observe at 4x and 10x magnification.
3. Drop NaCl solutions on the right side and absorb water on the left.
4. Wait and observe changes under the microscope.

Question 4

Methodology of observing osmosis in Chlorella vulgaris cells?

Answer 4

1. Prepare test tubes with Chlorella vulgaris culture and label them.
2. Add NaCl solutions and distilled water to separate tubes.
3. Shake the mixtures until homogeneous.
4. Drop one drop from each test tube on different glass slides, cover with slips, and observe under 4x and 10x magnification.

Question 5

effect of NaCl concentration on Rhoeo discolor?

Answer 5

1% NaCl: No significant change
5% NaCl: Some plasmolysis, cell membrane detachment, and shrinking
10% NaCl: More noticeable plasmolysis and membrane detachment from the cell wall. smaller and shriveled appearance

Question 6

effect of distilled water and NaCl concentrations on Chlorella vulgaris?

Answer 6

B (2 ml distilled water): Slight swelling, cells slightly turgid.
C (4 ml distilled water): Cells more turgid, larger due to water intake.
D (2 ml 10% NaCl): Cells separate, shrink due to plasmolysis (hypertonic).
E (4 ml 10% NaCl): More severe shrinking and separation from high salt concentration