Isolation of Casein and Separation of Amino Acids Using Paper Chromatography

Question 1

are essential constituents of all living cells and are vital for proper cellular structure and function

Answer 1

proteins

Question 2

are polymers composed of amino acid units joined by amide linkages

Answer 2

proteins

Question 3

proteins are polymers composed of amino acid units joined by

Answer 3

amide linkages

Question 4

are not only the building blocks of proteins but also occur in an uncombined form in numerous foods

Answer 4

amino acids

Question 5

are colorless, and their migration on paper cannot be followed visually

Answer 5

amino acids

Question 6

appear as white crystalline solids soluble in cold water except cystine and tyrosine which are soluble in hot water

Answer 6

amino acids

Question 7

amino acids appear as white crystalline solids soluble in cold water except _____________________ which are soluble in __________________

Answer 7

cystine and tyrosine; hot water

Question 8

Amino acids are insoloble in alcohol except

Answer 8

proline and hydroxyproline

Question 9

most of amino acids are

Answer 9

sweet

Question 10

is tasteless

Answer 10

leucine

Question 11

is bitter

Answer 11

arginine

Question 12

the separation and the isolation of various amino acids in the mixture after hydrolysis of a protein involves a complicated chemical procedure. The briefest outline usually involves an attept to first separate into groups such as the

Answer 12

• monoamino acids
• the basic acids (two amino groups)
• dicarboxylic acids (two carboxy)

Question 13

will extract many of the monoamino acids

Answer 13

butyl alcohol

Question 14

the basic amino acids are precipitated with

Answer 14

phosphotungstuc acid

Question 15

amino acids may be determined quantitatively by:

Answer 15

1. Colorimetric Methods
2. Microbiological Methods
3. Isotope dilution methods
4. Chromatographic analysis

Question 16

is a technique employed by chemists to separate or/and identify components of a micture

Answer 16

Chromatography

Question 17

The chromatography methods may be categorized into the following:

Answer 17

1. Column Chromatography
2. Paper Chromatography
3. Thin-layer Chromatography

Question 18

a strip of suitable paper is spotted at one end with the solution of the compound or mixture to be chromatographed

Answer 18

Paper Chromatography

Question 19

the spot is called the

Answer 19

origin

Question 20

in paper chromatography, a liquid called _________________ is allowed to move up a strip of paper by capillary action

Answer 20

mobile phase