ISOLATION AND ANALYSIS OF DNA FROM STRAWBERRY

Question 1

What is the contents of the homogenizing solution?

Answer 1

1. Ethanol
2. NaCl
3. Protease
4. SDS
5. Tris- Buffer

Question 2

Facilitates Na+ interaction with DNA’s phosphate groups.

Answer 2

Ethanol

Question 3

Na+ binds with DNA forming a precipitate (salting out method).

Answer 3

NaCl

Question 4

Breaks down proteins bound to DNA (better DNA yield).

Answer 4

Protease

Question 5

Breaks down the phospholipid bilayer of the cell particularly that of the nucleus (nuclear membrane) and the proteins in it, allowing for the release of DNA.

Answer 5

SDS

Question 6

Resuspension buffer making the DNA stble and viable

Answer 6

Tris-Buffer

Question 7

Purity of DNA: A260nm/A280nm

Answer 7

1.80 - 2.0

Question 8

Methods of detection of DNA and RNA concentration

Answer 8

a. Linear Regression
b. Optical Density
c. Densitometry

Question 9

Optical Density: Concentration (µg/ml) =

Answer 9

(A260 reading - A320 reading) x dilution factor x 50µg/ml

Question 10

Total yield is obtained by multiplying the

Answer 10

DNA concentration by the final total purified sample volume.

Question 11

DNA yield (microorganism /ml µg) =

Answer 11

DNA concentration × total sample volume (ml)

Question 12

Purpose of DNA extraction

Answer 12

a. Indetification
b. Genetic Disorders
c. Environmental Studies

Question 13

Other specimens for DNS extraction:

Answer 13

Human peripheral blood, buccal cell, hair follicle, urine and organs; plants animals and bacterial samples.

Question 14

indeed candidate sources of genes of medical or industrial importance.

Answer 14

DNA extract

Question 15

DNA isolation

Other techniques could be emplyed to harness this potential.

Answer 15

1. Polymerase chain reaction (PCR)
2. Purification steps
3. Sequencing (Bioinformatics)
4. Recombinant DNA technology
   ( restriction digestion, Ligation)

Question 16

DNA ISOLATION

Ethical consideration regarding extraction of DNA from __________

Answer 16

human source

Question 17

DNA ISOLATION

Ethical consideration regarding extraction of DNA from human source.
1.
2.
3.

Answer 17

1. Participants properly and clearly informed about the study.
2. Informed consent
3. Participants is informed about the outcome
4. Interventions

Question 18

DNA ISOLATION

Participants properly and clearly informed about the study.
1.
2.
3.

Answer 18

1. Background of the study
2. Protocol
3. Possible adverse effects

Question 19

2 Types of possbile adverse effects

Answer 19

1. Method of extraction
2. Exposure to reagents

Question 20

Informed consents

Answer 20

1. knowledge and permission that a biological sample is to be taken.
2. If sample will be stored at a given period of time or not
3. If further experimentations to be done using sample in the future.

Question 21

APPLICABILITY OF THE METHOD USED IN OTHER BIOLOGICAL SAMPLES.

Answer 21

1. Not an absolute method but provides the basic steps.
2. Modification are needed depending on the nature of sample source.
3. Possible sources of DNA and considerations.