is Flashcards
1) Who developed the technology of hybridoma cells used in the production of Monoclonal
antibodies?
a. Kohler & Milstein
b. Susumu Tonegawa
c. Joseph Marvin
d. Rodney Porter
a
2) What is the Process of monoclonal antibody production?
a. Stimulation of activated T cells
b. Fusion of plasma ell secretions
c. Fusion of antibody specific lymphocyte & myeloma cell
d. Multiplication of cytotoxic T cells
c
3) Antibodies produces against two or more epitopes of specific antigen are:
a. Monoclonal
b. Pleomorphic
c. Dimorphic
d. Polyclonal
d
4) In monoclonal antibody preparation what is the purpose of the HAT medium?
a. To be enriched with human AB serum
b. Tritiated thymidine is added to the culture media to allow growth of the myeloma cells
c. The medium allows Hybridoma cells to grow selectively in tissue culture
c
5) Which class of antibody can agglutinate RBCs after anti-human globulin (AHG) is added to the
test tube?
a. IgA
b. IgE
c. IgG
d. IgM
c
6) Function of AHG as a potentiator?
a. Directly reduces the ionic cloud surrounding RBCs
b. Promote bridging and rearrangement of antigen & antibody form lattice.
c. Acts on antigens to enhance their reactivity
b
7) Two biologically functional characteristics f immunoglobulin G (IgG) are:
a. First effective defense against bacteria; activation of the classical complement pathway
b. Opsonization of antigens for phagocytosis by macrophages and neutrophils; neonatal
immunity by transfer of maternal antibodies across the placenta & gut of fetus
c. Mucosal immunity, defends external body surfaces
d. Provides protection against parasitic helminthic infection; mediates the release of
histamines & heparin from mast cells & basophils.
b
8) Which of the following statement is false about an anamnestic/secondary response versus
primary response?
a. Has a shorter lag phase
b. Antibodies decline more gradually
c. Has a longer plateau
d. IgM antibodies predominate
d
9) Which T lymphocyte expresses the CD8+ marker and acts specifically to kill tumor or virally
infected cells?
a. Helper T lymphocytes
b. T reg lymphocytes
c. Natural Killer
d. T cytotoxic
d
10) The interaction between antigen presenting cells & T-helper cells is mediated by surface
expressed antigen and:
a. Interferon gamma
b. IL2
c. MHC class I molecule
d. MHC class II molecule
d
11) Alpha 1 antitrypsin:
a. Act as enzyme converting toxic ferrous ion to non-toxic ferric form
b. Promotes phagocytosis by binding to specific receptors found in monocyte macrophage
& neutrophil
c. Counteracts the effect of neutrophil invasion during inflammatory response
d. Act as antioxidant to provide protection against oxidative damage mediated by free
hemoglobin
c
12) A cluster of differentiation that is a part of the T cell antigen receptor complex.
a. CD 2 – receptor for sheep rbc / classical t cell surface marker
b. CD 3 – part of t cell antigen receptor complex
c. CD 4 – receptor for MHC class II
d. CD 8 – receptor for MHC class I
b
13) Which one of the following sets of cells can present antigen to helper T cells?
a. B cells & dendritic cells
b. B cells & cytotoxic T cells
c. Macrophages & eosinophils
d. Neutrophils & cytotoxic T cells
a
14) Which of the flowing is the receptor for the fragment crystallizable portion or nonspecific end,
of the immunoglobulin molecule IgG to Natural Killer Cell or Large Granular Lymphocyte?
a. CD 16
b. CD 56
c. CD 21 – B cells
d. CD 96 – not usually encountered
a
15) What density gradient is used to effectively isolate lymphocytes from rbcs?
a. Hank’s PBS
b. NaOH
c. Ficoll Hypaque
d. NSS
c
16) What is the second layer (from the top_ is Ficoll-Hypaque solution after centrifugation?
a. Plasma
b. Ficoll-Hypaque
c. Mononuclear cell
d. Granulocyte
c
17) Which of the following antibodies will immediately be induced should an intranasal covid-19
vaccine be administered?
a. IgG
b. IgE
c. IgD
d. IgA
d
18) How can you differentiate cytotoxic T cells (TC cells) from natural killer (NK)?
a. Able to release perforins & granzymes – both can release
b. Effective against virally infected cells
c. Recognize antigen in association with HLA class I
d. Do not bind to infected cells.
c
19) Which of the following precursor T-cells would not survive the selection process in the thymus
and eventually become mature T cells?
a. Thymocytes that have very high affinity for self-MHC antigens
b. Able to recognize foreign antigens along with MHC
c. Cannot bind to self-antigens other than those complexed with MHC
d. With functional TCR
a
20) Lymphocyte precursors that entered the thymus undergoes a significant selection process as it
matures and an estimated ____& of the cells die intrathymically before becoming mature T
cells.
a. 95%
b. 3%
c. 97%
d. 80-90
c
21) A cell that is identified by rosette formation with surface rbcs
a. T cell
b. B cell
c. NK cells
d. None of these
a
22) Which statement best describes passive agglutination reactions used for serodiagnosis?
a. Such agglutination reactions are more rapid because they are a single-step process
b. Reactions require the addition of a second antibody
c. Passive agglutination reactions require biphasic incubation
d. Carrier particles for antigen such as latex particles are used
d
23) What is the main purpose of incorporating latex in the CRP reagent?
a. To facilitate better visualization under the microscope
b. To make the antibody insoluble and particulate
c. Will serve as the antigen carrier
d. To have a color contrast
b
24) The test circle for the positive control for CRP determination should contain?
a. CRP latex reagent + human serum
b. CRP latex reagent + positive control
c. Positive control + human serum
d. CRP latex reagent + negative control
b
25) Cathepsin G & defensins are involved in the process of?
a. Positive chemotaxis
b. Initiating attachment of pathogens to toll like receptors
c. Creation of punches and holes to target cells to facilitate entry of granzymes
d. Oxygen-independent digestion
c
26) Which type of antigen is processed by immunocompetent cells for presentation via MHC class II
a. Exogenous peptides
b. Endogenous peptides
c. Both
d. Neither
a
27) Which of the following best describes the role of Tap?
a. They bind to class II molecules to help block the antigen binding site
b. They bind to class I proteins in proteasomes
c. They transport peptides into the lumen of the endoplasmic reticulum
d. They help cleave peptides for transport to endosomes
c
28) O cells acts as a control upon retesting in what cases would this test be positive?
I. normal serum of blood type O (no anti-H = negative)
II. Rouleaux formation (false positive)
III. Bombay Phenotype (has anti-H = strong pos reaction)
IV. Serum of blood type AB (no anti-H,=negative)
A. I,II
B. I,II,III
C. II, III
D. all of the above
c
9) Immunoglobulins detected on serum of newborn patients are typically of what type?
a. Maternal IgM
b. Maternal IgG
c. Neonatal IgG
d. Placental IgM
b
30) What type of agglutination is ABH secretor study?
a. Direct
b. Indirect
c. Reverse passive
d. Agglutination inhibition
d
31) . Interpret the blood group and secretor status of a patient based on the panel below:
D
32) Humoral immunity is mediated by antibodies from __ & is involved in the elimation of ___
pathogens. Cell mediated immunity is mediated by ___ & is involved in the elimination of ___
pathogens.
a. B-cells; Intracellular: T-cells; Extracellular
b. B-cells; Extracellular: T-cells; Intracellular
c. T-cells; Intracellular: B-cells; Extracellular
d. T-cells; Extracellular: B-cells; Intracellular
b
33) Within what period of time after performance of the tuberculin skin test (TST) should the result
be read?
a. Immediately
b. 12-24 hrs
c. 48-72 hrs
d. 5-7 days
c
34) While serum elevations are NOT generally seen in early stages, which of the ff tumor markers
are elevated in more advanced stages of breast cancer
a. CEA & AFP
b. AFP & CA125
c. PSA & CA 15-3
d. CA 15-3 & CA 549
d
35) All of the ff are associated with immediate hypersensitivity except?
a. Hay fever
b. Urticaria (Hives)
c. Tuberculin reaction
d. Anaphylactic shock
c
36) Which complement pathway & related initiator can activate complement
a. Classic pathway; antigen-IgG antibody complexes
b. Classic pathway; tumor cells
c. Alternative pathway; certain viruses & gram-negative bacteria
d. Mannose-Lectin pathway; apoptotic cells
a
37) The most highly specific indicator for rheumatoid arthritis is:
a. Rheumatoid factor
b. Cyclic citrullinated peptide antibodies (CCP antibodies) = anti CCP
c. Immune complexes
d. Complement
b
38) A physician suspects his patient might have rheumatoid arthritis. Which of the ff markers should
be ordered?
a. Antimitochondrial antibodies
b. Anti-IgG
c. Antineutrophilic antibodies
d. Antimyocardial antibodies
b
39) Which of the ff best describes the slide agglutination test for RF?
I. specific for SLE
II. a negative test rules out possiblity of RA
III. it is a sensitive screening tool
IV. it detects IgM made against IgG
a. I, II, II
b. III, IV
c. I, III
d. I, II
b