Investigative Techniques

Question 1

What are the requirements to image tissue by light microscopy?

Answer 1

Preserve
Embed
Stain

Question 2

How can tissue be preserved?

Answer 2

Formalin

Question 3

What can tissue be embedded in?

Answer 3

Melted paraffin

Question 4

How thin should tissue be for light microscopy?

Answer 4

5 microns - using a microtome

Question 5

What does harmatoxylin stain do?

Answer 5

Stains nucleus blue

Question 6

What does eosin stain do?

Answer 6

Stains cytoplasm pink

Question 7

In frozen sections to what temp should tissue be frozen to and how?

Answer 7

-20 to -30 via cryostat

Question 8

Advantages and disadvantages of frozen section

Answer 8

Quick

Lower quality

Question 9

What technique filters light in only one direction?

Answer 9

Polarised light microscopy

Question 10

What is the problem causing gout

Answer 10

Too much urate

Question 11

What treatment does gout respond well to?

Answer 11

NSAIDs

Question 12

What crystals are present in gout?

Answer 12

Monosodium urate

Question 13

What do monosodium urate crystals look like?

Answer 13

Negative birefringence needles

Question 14

What crystals are present in pseudogout?

Answer 14

Calcium pyrophosphate dihydrate

Question 15

Advantages of confocal microscopy

Answer 15

Live specimens
Time lapse

(use a pinhole)

Question 16

What is the resolution and magnification of modern light microscopy?

Answer 16

x1000

0.2 micron

Question 17

Disadvantage of electron microscopy

Answer 17

Dead tissue as uses a vacuum

Question 18

Magnification of EM

Answer 18

x250000

Question 19

What can freeze fracture EM be used for?

Answer 19

Fracture line goes through the PM exposing the interior to be imaged

Question 20

What can you study using SEM

Answer 20

SUrface