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Chemical Technician (Analytical Chemistry) > Introduction to Chromatography > Flashcards

Introduction to Chromatography Flashcards

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1
Q
  1. Chromatography is a physical method that is used to
    separate and analyse __________
    a) Simple mixtures
    b) Complex mixtures
    c) Viscous mixtures
    d) Metals
A

b) Complex mixtures

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2
Q
  1. In which type of chromatography, the stationary phase
    held in a narrow tube and the mobile phase is forced
    through it under pressure?
    a) Column chromatography
    b) Planar chromatography
    c) Liquid chromatography
    d) Gas chromatography
A

a) Column chromatography

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3
Q
  1. In chromatography, the stationary phase can be
    ___________ supported on a solid.
    a) Solid or liquid
    b) Liquid or gas
    c) Solid only
    d) Liquid only
A

a) Solid or liquid

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4
Q
  1. In chromatography, which of the following can the
    mobile phase be made of?
    a) Solid or liquid
    b) Liquid or gas
    c) Gas only
    d) Liquid only
A

b) Liquid or gas

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5
Q
  1. Which of the following cannot be used as an adsorbent
    in Column adsorption chromatography?
    a) Magnesium oxide
    b) Silica gel
    c) Activated alumina
    d) Potassium permanganate
A

d) Potassium permanganate

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6
Q
  1. Which of the following types of chromatography
    involves the separation of substances in a mixture over a
    0.2mm thick layer of an adsorbent?
    a) Gas liquid
    b) Column
    c) Thin layer
    d) Paper
A

c) Thin layer

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7
Q
  1. Chromatography cannot be used to purify volatile
    substances.
    a) True
    b) False
A

b) False

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8
Q
  1. In Column chromatography, the stationary phase is
    made of _________ and the mobile phase is made of
    _________
    a) Solid, liquid
    b) Liquid, liquid
    c) Liquid, gas
    d) Solid, gas
A

a) Solid, liquid

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9
Q
  1. Chromatography cannot be used to separate delicate
    products.
    a) True
    b) False
A

b) False

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10
Q
  1. In Thin layer chromatography, the stationary phase is
    made of _________ and the mobile phase is made of
    _________
    a) Solid, liquid
    b) Liquid, liquid
    c) Liquid, gas
    d) Solid, gas
A

a) Solid, liquid

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11
Q
  1. In Gas-liquid phase chromatography, the stationary
    phase is composed of _________ and the mobile phase is
    made of _________
    a) Solid, liquid
    b) Liquid, liquid
    c) Liquid, gas
    d) Solid, gas
A

c) Liquid, gas

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12
Q
  1. In which of the following type of paper,
    chromatography does the mobile phase move horizontally
    over a circular sheet of paper?
    a) Ascending paper chromatography
    b) Descending paper chromatography
    c) Radial paper chromatography
    d) Ascending – descending chromatography
A

c) Radial paper chromatography

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12
Q
  1. Liquid chromatography can be performed in which of
    the following ways?
    a) Only in columns
    b) Only on plane surfaces
    c) Either in columns or on plane surfaces
    d) Neither in columns nor on plane surfaces
A

c) Either in columns or on plane surfaces

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13
Q
  1. Gas chromatography can be performed in which of the
    following ways?
    a) Only in columns
    b) Only on plane surfaces
    c) Either in columns or on plane surfaces
    d) Neither in columns nor on plane surfaces
A

a) Only in columns

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14
Q
  1. Which of the following types of chromatography
    involves the process, where the mobile phase moves
    through the stationary phase by the influence of gravity or
    capillary action?
    a) Column Chromatography
    b) High Pressure Liquid Chromatography
    c) Gas Chromatography
    d) Planar Chromatography
A

d) Planar Chromatography

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15
Q
  1. Which of the following steps takes place after injection
    of feed in Column chromatography?
    a) Detection of components
    b) Separation in the column
    c) Elution from the column
    d) Collection of eluted component
A

a) Detection of components

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16
Q
  1. What happens during the ‘elution from the column’
    phase in chromatography?
    a) Components with greatest affinity elute first
    b) Components with least affinity elute first
    c) Components elute in a random manner
    d) Components elute according to their concentration in
    the mixture
A

b) Components with least affinity elute first

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17
Q
  1. Components with a strong attraction to the support
    move more slowly than components with weak attraction.
    a) True
    b) False
A

a) True

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18
Q
  1. In chromatogram, the position of peaks on the time axis
    can be used to determine which of the following?
    a) Components of the sample
    b) Amount of component in the sample
    c) Column efficiency
    d) Column resolution
A

a) Components of the sample

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18
Q
  1. In chromatogram, the area under the peak can be used
    to determine which of the following?
    a) Components of the sample
    b) Amount of component in the sample
    c) Column efficiency
    d) Column resolution
A

b) Amount of component in the sample

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19
Q
  1. The stationary phase could be a viscous liquid coated
    over a surface of solid particles.
    a) True
    b) False
A

a) True

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20
Q
  1. Using Chromatogram as detector in Chromatography, a
    graph is obtained between ____________ and time.
    a) Quantity
    b) Density
    c) Concentration
    d) Specific gravity
A

c) Concentration

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21
Q
  1. How is the molar concentration of solute in stationary
    phase related to molar concentration of solute in the
    mobile phase?
    a) Directly proportional
    b) Inversely proportional
    c) Equal
    d) Not related
A

a) Directly proportional

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21
Q
  1. In older analytical methods, which of the following
    methods were used to allow movement of the mobile
    phase?
    a) Pumps
    b) Pressure
    c) Gravity
    d) Blowing air into the column
A

c) Gravity

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22
Q
  1. If the value of the distribution constant ‘k’ is one, then
    what could be inferred about the distribution of solute?
    a) Its distribution in stationary phase is greater
    b) Its distribution in mobile phase is greater
    c) It is equally distributed in stationary and mobile phase
    d) It is distributed in a random manner
A
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23
Q
  1. The time taken by the analyte after sample injection to
    reach the detector is called _________
    a) Dead time
    b) Solute migration rate
    c) Adjusted retention time
    d) Retention time
A

d) Retention time

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24
Q
  1. The time required for a molecule of the mobile phase to
    pass through the column is called ___________
    a) Dead time
    b) Solute migration rate
    c) Adjusted retention time
    d) Retention time
A

a) Dead time

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25
Q
  1. Adjusted retention time is the remaining retention time
    after subtracting __________ from ___________
    a) Solute migration rate and retention time
    b) Retention time and solute migration rate
    c) Dead time and retention time
    d) Retention time and dead time
A

c) Dead time and retention time

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26
Q
  1. Which of the following is the volume of mobile phase
    required to make a solute band move from the point of
    injection through the column to the detector?
    a) Dead volume
    b) Retention volume
    c) Void volume
    d) Adjusted retention volume
A

b) Retention volume

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27
Q
  1. Adjusted retention volume is the remaining retention
    volume after subtracting ___________ from
    _____________
    a) Solute migration rate and retention volume
    b) Retention volume and solute migration rate
    c) Dead volume and retention volume
    d) Retention volume and dead volume
A

c) Dead volume and retention volume

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28
Q
  1. Which of the following is defined as the ratio of moles of
    solute in stationary phase to the moles of solute in the
    mobile phase?
    a) Distribution constant
    b) Volumetric phase ratio
    c) Retention factor
    d) Total porosity
A

c) Retention factor

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29
Q
  1. Which of the following is the ratio of the interstitial
    volume of packing to the volume of its total mass?
    a) Distribution constant
    b) Volumetric phase ratio
    c) Retention factor
    d) Total porosity
A

d) Total porosity

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30
Q
  1. Which of the following is the ratio of the length of
    column packing to dead time?
    a) Average linear rate of solute migration
    b) Average linear rate of mobile migration
    c) Relative migration rate
    d) Selectivity factor
A

b) Average linear rate of mobile migration

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31
Q
  1. Which of the following is the ratio of a length of
    column packing to retention time?
    a) Average linear rate of solute migration
    b) Average linear rate of mobile migration
    c) Relative migration rate
    d) Selectivity factor
A

a) Average linear rate of solute migration

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32
Q
  1. Retention distance is the distance between point of
    injection and minimum peak in the recorder or computer
    generated chart.
    a) True
    b) False
A

b) False

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33
Q
  1. Retention factor is also known as the capacitance
    factor.
    a) True
    b) False
A

a) True

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34
Q
  1. Retention volume can be obtained by finding the
    product of which of the following parameters?
    a) Dead time and total porosity
    b) Retention time and volumetric flow rate
    c) Adjusted retention time and volumetric flow rate
    d) Retention time and total porosity.
A

b) Retention time and volumetric flow rate

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35
Q
  1. What must be the value of the selectivity factor?
    a) Equal to 1
    b) Less than 1
    c) Greater than 1
    d) Greater than 0
A

c) Greater than 1

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36
Q
  1. Which of the following is the distance that the solute
    moves while undergoing one partition?
    a) Retention distance
    b) Distribution constant
    c) Plate height
    d) Column packing length
A

c) Plate height

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37
Q
  1. Which of the following conditions will cause the
    efficiency of the column to increase?
    a) Plate number becomes greater, plate height becomes
    smaller
    b) Plate number becomes smaller, plate height becomes
    smaller
    c) Plate number becomes greater, plate height becomes
    larger
    d) Plate number becomes greater, plate height becomes
    larger
A

a) Plate number becomes greater, plate height becomes
smaller

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38
Q
  1. Which of the following is the expression for Eddy
    diffusion in the column, if ‘γ’ represents obstruction factor,
    ‘dp’ represents particle diameter, ‘DM’ represents Solute
    diffusion co-efficient and ‘λ’ represents function for
    packing uniformity?
    a) λ dM
    b) 2 γ DM
    c) DM γ
    d) λ/dM
A

a) λ dM

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39
Q
  1. Which of the following is the expression for longitudinal
    diffusion in the column, if ‘γ’ represents obstruction factor,
    ‘dM’ represents particle diameter, ‘DM’ represents Solute
    diffusion co-efficient and ‘λ’ represents function for
    packing uniformity?
    a) λ dM
    b) 2 γ DM
    c) DM γ
    d) λ/dM
A

b) 2 γ DM

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40
Q
  1. In gas-liquid chromatography, when films are used in
    the interior of the capillary column, then what is the value
    of Eddy diffusion?
    a) Greater than 1
    b) Less than 1
    c) Zero
    d) Less than zero
A

c) Zero

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41
Q
  1. For an unpacked coated capillary column, the
    obstruction factor takes which of the following values?
    a) 0
    b) 0.6
    c) 1
    d) 1.6
A

c) 1

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42
Q
  1. Base line resolution is achieved when degree of
    resolution/separation is ______
    a) 1
    b) 0
    c) 0.5
    d) 1.5
A

d) 1.5

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43
Q
  1. Which of the following equations give the expression for plate number, N when ‘tR’ is the adjusted retention time and ‘Wb’ is the width at the base of the peak which is
    equal to 4 standard deviations.
    a) 16 tR
    2/Wb
    b) 4 tR
    2/Wb
    c) (4tR/Wb)^2
    d) 4 (tR/Wb)2
A

c) (4tR/Wb)^2

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44
Q
  1. For the separation of which of the following substances,
    Gas-solid chromatography is being used?
    a) Thermally stable organic components
    b) Volatile organic components
    c) Thermally stable inorganic components
    d) Low molecular weight gaseous species
A

d) Low molecular weight gaseous species

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44
Q
  1. It is more difficult to measure the width at half peak
    height than the base width in the detector output.
    a) True
    b) False
A

b) False

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44
Q
  1. The solute, while moving in the column is always in
    equilibrium with both the stationary phase and the mobile
    phase.
    a) True
    b) False
A

a) True

45
Q
  1. Which of the following is not a feature of carrier gas
    used in gas chromatography?
    a) It must be chemically inert
    b) It should be suitable for the detector employed
    c) It should not be completely pure
    d) It should be cheap
A

c) It should not be completely pure

46
Q
  1. Which of the following is the disadvantage of hydrogen,
    which can be used as carrier gas in gas chromatography?
    a) Dangerous to use
    b) Expensive
    c) Reduced sensitivity
    d) High density
A

a) Dangerous to use

47
Q
  1. Which of the following is the disadvantage of helium,
    which can be used as carrier gas in gas chromatography?
    a) Dangerous to use
    b) Expensive
    c) Reduced sensitivity
    d) High density
A

b) Expensive

48
Q
  1. Which of the following is the disadvantage of nitrogen,
    which can be used as carrier gas in gas chromatography?
    a) Dangerous to use
    b) Expensive
    c) Reduced sensitivity
    d) High density
A

c) Reduced sensitivity

49
Q
  1. Slow injection of large samples leads to band
    broadening and loss of resolution.
    a) True
    b) False
A

a) True

50
Q
  1. In which of the following methods are liquid samples
    injected into the column in gas chromatography?
    a) Gas tight syringe
    b) Micro-syringe
    c) Rotary sample valve
    d) Solid injection syringes
A

b) Micro-syringe

51
Q
  1. Which of the following is the commonly used support
    material for the packed column in gas chromatography?
    a) Glass
    b) Metal
    c) Diatomaceous earth
    d) Stainless steel
A

c) Diatomaceous earth

52
Q
  1. What must be done to the solid samples for it to be
    introduced into the column without using solid injection
    syringes in gas chromatography?
    a) Introduced in hot-zone of the column
    b) Dissolved in volatile liquids
    c) Introduced using rotary sample valve
    d) Introduced using sampling loop
A

b) Dissolved in volatile liquids

53
Q
  1. Sample injection port must be maintained at a
    temperature at which rapid vapourisation occurs but
    thermal degradation does not occur.
    a) True
    b) False
A

a) True

53
Q
  1. Which of the following is the disadvantage of coiled or
    helical shaped packed chromatographic column?
    a) It cannot be packed uniformly
    b) It cannot be repacked easily
    c) It is not compact
    d) It is not easy to heat it evenly
A

b) It cannot be repacked easily

54
Q
  1. Capillary columns are open tubular columns
    constructed from which of the following materials?
    a) Glass
    b) Metal
    c) Stainless steel
    d) Fused silica
A

d) Fused silica

54
Q
  1. Which of the following is the advantage of a straight
    packed column?
    a) It can be packed uniformly
    b) It can be repacked easily
    c) It is compact
    d) It is easier to heat it evenly
A

c) It is compact

55
Q
  1. Which of the following is not a desirable feature of the
    ovens used in gas chromatography?
    a) It must have a fast rate of heating
    b) Power consumption should be kept low
    c) It must have maximum thermal gradients
    d) It should have proper insulation
A

c) It must have maximum thermal gradients

56
Q
  1. Which of the following is not an ideal characteristic of a
    detector used in gas chromatography?
    a) Linear response to the solutes
    b) Short response time
    c) High reliability
    d) Sensitive to the changes in the flow rate of a carrier gas
A

d) Sensitive to the changes in the flow rate of a carrier gas

57
Q
  1. Which of the following detectors have high sensitivity to
    all organic compounds?
    a) Sulphur chemiluminescense detector
    b) Thermionic emission detector
    c) Flame ionization detector
    d) Argon ionisation detector
A

c) Flame ionization detector

58
Q
  1. Which of the following is not a type of detector used in
    gas chromatography?
    a) Argon ionisation detector
    b) Thermal conductivity detector
    c) UV visible spectrometric detector
    d) Electron capture detector
A

c) UV visible spectrometric detector

59
Q
  1. Which of the following is not the advantage of thermal
    conductivity detector used in gas chromatography?
    a) Simple in construction
    b) High sensitivity
    c) Large linear dynamic range
    d) Non-destructive character
A

b) High sensitivity

60
Q
  1. Which of the following detectors is widely used to
    detect environmental samples like chlorinated pesticides
    and polychlorinated biphenyls?
    a) Flame ionization detector
    b) Thermal conductivity detector
    c) Argon ionisation detector
    d) Electron capture detector
A

d) Electron capture detector

61
Q
  1. In which of the following detector is the eluent mixed
    with hydrogen and burned and then mixed with ozone and
    its intensity is measured?
    a) Sulphur chemiluminescense detector
    b) Thermal conductivity detector
    c) Flame ionization detector
    d) Electron capture detector
A

a) Sulphur chemiluminescense detector

62
Q
  1. Which of the following detector uses ultraviolet
    radiation from lamps to produce ionisation of solute
    molecules?
    a) Sulphur chemiluminescense detector
    b) Thermal conductivity detector
    c) Photo ionization detector
    d) Electron capture detector
A

c) Photo ionization detector

62
Q
  1. Flame ionisation detector is also known as
    Katharometer.
    a) True
    b) False
A

b) False

62
Q
  1. Filter photometer detector is primarily responsive to
    which of the following compounds/elements?
    a) Volatile sulphur or phosphorous compounds
    b) Nitrogen
    c) Halogen
    d) Potassium
A

a) Volatile sulphur or phosphorous compounds

63
Q
  1. Thermionic emission detector used in gas
    chromatography is most sensitive to which of the
    following elements?
    a) Nitrogen
    b) Phosphorous
    c) Halogen
    d) Carbon
A

b) Phosphorous

64
Q
  1. Which of the following detectors has a non-volatile
    bead of rubidium silicate placed above the flame tip?
    a) Argon ionisation detector
    b) Thermionic emission detector
    c) Flame ionization detector
    d) Electron capture detector
A

b) Thermionic emission detector

64
Q
  1. In which of the following detectors, the carrier gas is
    excited by a radioactive source and the atoms of carrier
    gas are excited to a metastable state?
    a) Argon ionisation detector
    b) Thermionic emission detector
    c) Flame ionization detector
    d) Electron capture detector
A

a) Argon ionisation detector

65
Q
  1. Which of the following is not used as a heating
    element in a Thermal conductivity detector?
    a) Platinum
    b) Gold
    c) Graphite
    d) Tungsten wire
A

c) Graphite

66
Q
  1. Gas-solid chromatography is based on which of the
    following processes?
    a) Partition of the analyte between a gaseous mobile
    phase and a stationary liquid phase
    b) Adsorption of gaseous substances on solid surface
    c) Ion exchange
    d) Large molecules cannot penetrate through the gel
A

b) Adsorption of gaseous substances on solid surface

66
Q
  1. Electron capture detector is much less susceptible to
    contamination when nickel is used instead of tritium.
    a) True
    b) False
A

a) True

67
Q
  1. Which of the following components cannot be retained
    by gas-liquid columns but can be separated by using gas
    solid chromatography?
    a) Formaldehyde
    b) Hydrogen sulphide
    c) Benzene
    d) Carbon dioxide
A

b) Hydrogen sulphide

68
Q
  1. Which of the following is not an advantage of gas-solid
    chromatography?
    a) Increased column life
    b) Can be used for separation of rare gases
    c) Leads to semi-permanent retention of the analyte
    d) Ability to retain some components that cannot be easily
    retained by other gas chromatography method
A

c) Leads to semi-permanent retention of the analyte

69
Q
  1. The distribution coefficients of Gas-solid
    chromatography are greater than that of Gas-liquid
    chromatography.
    c
    b) False
A

a) True

70
Q
  1. Which of the following columns can be used in Gas-solid
    chromatography?
    a) Open tubular column
    b) Analytical column
    c) Separation column
    d) Guard column
A

a) Open tubular column

71
Q
  1. Which of the following is not an advantage for the
    conversion of packed columns into wide bore capillaries?
    a) Longer retention times
    b) Longer life
    c) Higher efficiency
    d) Greater inertness
A

a) Longer retention times

72
Q
  1. Which of the following is not a disadvantage of gas-solid
    chromatography?
    a) Strong retention of polar solutes
    b) Lifetime is short
    c) Occurrence of catalytic changes
    d) Cannot be used for very wide range of components
A

b) Lifetime is short

73
Q
  1. Gas-solid chromatography can be used only for
    separation of certain low molecular weight gaseous
    species.
    a) True
    b) False
A

a) True

74
Q
  1. Which of the following is a special adsorbent used in
    gas-solid chromatography?
    a) Molecular sieves
    b) Silica gel
    c) Alumina
    d) Starch
A

a) Molecular sieves

75
Q
  1. Which of the following properties of molecular sieves
    make it ideal for exclusion mechanism of separation?
    a) High thermal stability
    b) Large inner surface area
    c) Variable framework charge
    d) Ability to distinguish materials on the basis of their size
A

d) Ability to distinguish materials on the basis of their size

76
Q
  1. Which of the following is the disadvantage of
    reciprocating pump used in liquid chromatography?
    a) Produces pulsed flow
    b) Corrosive components
    c) Does not have small hold-up value
    d) Does not have moderate flow rate
A

a) Produces pulsed flow

77
Q
  1. Which of the following is not a disadvantage of
    Pneumatic pumps used in liquid chromatography?
    a) Pulsed output
    b) Dependent on solvent viscosity
    c) Dependent on back pressure
    d) Inconvenient for solvent gradient elution
A

a) Pulsed output

78
Q
  1. Which of the following will improve the efficiency of the
    separation process in liquid chromatography?
    a) Increase in sample size, increase in column diameter
    b) Reduction in sample size, increase in column diameter
    c) Increase in sample size, reduction in column diameter
    d) Reduction in sample size, reduction in column diameter
A

d) Reduction in sample size, reduction in column diameter

78
Q
  1. Which of the following is not a desired characteristic of
    pulse dampers or flow smootheners used in liquid
    chromatography?
    a) Easy mobile phase change over
    b) Constant flow must be maintained
    c) Should be effective at low system pressure
    d) Maximal dead volume
A

d) Maximal dead volume

79
Q
  1. Which of the following are the practical problems that
    arise due to the decrease in column diameter?
    a) Requirement of large particle size and high pressure
    drop
    b) Requirement of large particle size and low pressure
    drop
    c) Requirement of small particle size and high pressure
    drop
    d) Requirement of small particle size and low pressure
    drop
A

c) Requirement of small particle size and high pressure
drop

80
Q
  1. Which of the following is not true about the guard
    column used in liquid chromatography?
    a) It filters particles that clog the separation column
    b) It extends the lifetime of separation column
    c) It allows particles that cause precipitation upon contact with stationary or mobile phase
    d) The size of packing varies with the type of protection
    needed
A

c) It allows particles that cause precipitation upon contact with stationary or mobile phase

81
Q
  1. Which of the following columns are not used in liquid or
    high performance liquid chromatography?
    a) Analytical column
    b) Separation column
    c) Guard column
    d) Capillary column
A

d) Capillary column

82
Q
  1. Which of the following is not a Column-type Liquid
    chromatography?
    a) Gel permeation
    b) Ion exchange
    c) Liquid-solid
    d) Paper
A

d) Paper

83
Q
  1. Which of the following is not true about the radial
    compression column when compared to standard
    separation column?
    a) Internal diameter decreases
    b) Overall operating pressure decreases
    c) Analysis time decreases
    d) Solvent flow increases
A

a) Internal diameter decreases

83
Q
  1. Which of the following is not true about narrow bore
    column when compared to standard columns?
    a) Internal diameter decreases
    b) Volumetric flow decreases
    c) Solvent cost is saved
    d) Detector response time increases
A

d) Detector response time increases

84
Q
  1. Which of the following types of liquid chromatography
    uses immobilized biochemical as a stationary phase?
    a) Ion exchange chromatography
    b) Exclusion chromatography
    c) Affinity chromatography
    d) Gel permeation chromatography
A

c) Affinity chromatography

85
Q
  1. Which of the following is not true about High pressure
    liquid chromatography (HPLC)?
    a) It requires high pressure for the separation of the
    specious
    b) There is no need to vaporise the samples
    c) It is performed in columns
    d) It has high sensitivity
A

b) There is no need to vaporise the samples

85
Q
  1. High pressure liquid chromatography can be performed
    only in columns.
    a) True
    b) False
A

a) True

86
Q
  1. Which of the following is not an advantage of Syringe
    type pumps used in High pressure liquid chromatography?
    a) Independent of viscosity
    b) Pulse-less flow
    c) High pressure capability
    d) Unlimited solvent capacity
A

d) Unlimited solvent capacity

87
Q
  1. Which of the following pulse damper takes up some
    amount of the pulsation energy which is released to
    provide smooth pressure without pulsations?
    a) Flexible bellows or compressible gas passed through tee columns
    b) Flexible inert diaphragm
    c) Electronic pulse damper
    d) Electrical pulse damper
A

a) Flexible bellows or compressible gas passed through tee columns

87
Q
  1. Which of the following is not true about solvent
    programming which is done in high performance liquid
    chromatography?
    a) It provides unequal bandwidths
    b) It provides fast overall separation
    c) It provides maximum resolution
    d) It provides maximum sensitivity
A

a) It provides unequal bandwidths

88
Q
  1. Syringe pumps used in High pressure liquid
    chromatography are most suitable for which of the
    following columns?
    a) Capillary columns
    b) Guard columns
    c) Short-fast columns
    d) Small bore columns
A

d) Small bore columns

89
Q
  1. Which of the following is not a characteristic of the
    syringe pump used in high pressure liquid
    chromatography?
    a) Pressure capability is high
    b) Maintenance is frequent
    c) Limited reservoir capability
    d) Slight change of flow rate when extremely high pressure
    compresses the solvent
A

b) Maintenance is frequent

90
Q
  1. Gravity feed method for solvent delivery is not used
    with narrow bore columns packed with fine mesh
    particles.
    a) True
    b) False
A

a) True

91
Q
  1. Which of the following cannot be done to reduce ripple
    in High pressure liquid chromatography?
    a) Using bellows
    b) Using restrictors
    c) Using long nylon tube between pump and column
    d) Avoiding the use of the solvent pump
A

d) Avoiding the use of the solvent pump

92
Q
  1. Which of the following is not true about Hydraulic
    capacitance flow control system used in HPLC?
    a) It can be used only for liquids with low viscosity
    b) It is irrespective of solvent compressibility
    c) It maintains a constant flow
    d) It smoothens high pressure pump pulsations
A

a) It can be used only for liquids with low viscosity

93
Q
  1. Bulk property detectors used in liquid chromatography
    does not respond to which of the following properties?
    a) Refractive index
    b) Density
    c) Properties of solutes
    d) Dielectric constant
A

c) Properties of solutes

94
Q
  1. Which of the following is not a property of a good
    detector used in liquid chromatography?
    a) Good sensitivity
    b) Ability to function in the presence of a large background
    signal
    c) Short response time
    d) Volume of detector must be large
A

d) Volume of detector must be large

94
Q
  1. Detector selectivity is more important in Liquid
    chromatography than in Gas chromatography.
    a) True
    b) False
A

a) True

95
Q
  1. Which of the following UV absorbance detectors
    provide a real time spectrum of the component of
    interest?
    a) Continuous wavelength detector
    b) Variable wavelength detector
    c) Scanning wavelength detector
    d) Fixed wavelength detector
A

c) Scanning wavelength detector

96
Q
  1. Fluorescence detection is less selective than absorption
    detection.
    a) True
    b) False
A

b) False

96
Q
  1. Which of the following detectors depend on Snell’s law
    at the interface between the cell wall and the flowing
    liquid to deflect the light beam?
    a) Electrochemical detectors
    b) Fluorescence detectors
    c) Refractive index detectors
    d) Thermal conductivity detectors
A

c) Refractive index detectors

97
Q
  1. Refractive index detectors used in liquid
    chromatography are not based on which of the following
    processes?
    a) Interference
    b) Refraction
    c) Reflection
    d) Absorption
A

d) Absorption

98
Q
  1. The reference cell is packed with which of the following
    in the Adsorption detector used in liquid chromatography?
    a) Inactive glass beads
    b) Porous glass beads
    c) Alumina
    d) Silica
A

a) Inactive glass beads

98
Q
  1. Which of the following detectors can be used for
    detection of amino acids in protein hydrolyzates by
    introducing the reagent dansylchloride in the sample?
    a) Electrochemical detectors
    b) Fluorescence detectors
    c) Refractive index detectors
    d) Thermal conductivity detectors
A

b) Fluorescence detectors

99
Q
  1. Which of the following is true about Laser detectors
    used in liquid chromatography?
    a) Causes thermal distortion has decreased sensitivity
    b) Causes thermal distortion has increased sensitivity
    c) Does not cause thermal distortion, has decreased
    sensitivity
    d) Does not cause thermal distortion, has increased
    sensitivity
A

a) Causes thermal distortion has decreased sensitivity

100
Q
  1. Which of the following detectors used in liquid
    chromatography is also called micro-adsorption detector?
    a) Electrochemical detectors
    b) Fluorescence detectors
    c) Refractive index detectors
    d) Thermal detectors
A

d) Thermal detectors

101
Q
  1. In UV-Visible detectors, the cells are not made of
    which of the following materials?
    a) Quartz
    b) Teflon
    c) Silica
    d) KELF
A

c) Silica

102
Q
  1. Which of the following is not a feature of refractive
    index type detectors?
    a) Higher potential sensitivity
    b) Low cost
    c) High volume flow rates
    d) Easy cell accessibility
A

c) High volume flow rates

103
Q
  1. Which of the following detectors can detect almost any
    component?
    a) Combining two UV detectors
    b) Combining RI and UV detectors
    c) Mass detector
    d) Laser-based detectors
A

b) Combining RI and UV detectors

Chemical Technician (Analytical Chemistry) (16 decks)

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