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Chemical Technician (Analytical Chemistry) > Chromatography > Flashcards

Chromatography Flashcards

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1
Q
  1. Chromatography is the process for identification,
    purification and separation of components of a mixture on
    the basis of
    a) Difference in their boiling point
    b) Difference in their melting point
    c) Difference in their affinity for mobile and stationary
    phase
    d) Difference in their solubility
A

c) Difference in their affinity for mobile and stationary
phase

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2
Q
  1. Chromatography involves two mutually
    a) Immiscible phases
    b) Miscible phases
    c) Soluble phases
    d) None of the mentioned
A

a) Immiscible phases

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3
Q
  1. There is no need of support if packed into a column
    when the stationary phase is
    a) Gas
    b) Liquid
    c) Solid
    d) None of the mentioned
A

c) Solid

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3
Q
  1. Which of the following is not stationary phase?
    a) Liquid-liquid chromatography
    b) Gas-liquid chromatography
    c) Gas-solid chromatography
    d) Solid-solid chromatography
A

d) Solid-solid chromatography

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4
Q
  1. Liquid chromatography is a technique for separating
    a) Ions that are not dissolved in a solvent
    b) Ions that are dissolved in a solvent
    c) Ions that are dissolved in a solute
    d) All of the mentioned
A

b) Ions that are dissolved in a solvent

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5
Q
  1. The gas-solid chromatography is ______________
    chromatography as per basic principle involved.
    a) Exclusion
    b) Ion-exchange
    c) Adsorption
    d) Absorption
A

c) Adsorption

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5
Q
  1. In exclusion chromatography, solute molecules are
    separated based on
    a) Molecular geometry and size
    b) Molecular composition
    c) Molecular phase
    d) Molecular formula
A

a) Molecular geometry and size

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6
Q
  1. A proper solvent that is passed through the column for
    elution so that separated components can be collected is
    called
    a) Adsorbent
    b) Buffer solution
    c) Mobile phase
    d) None of the mentioned
A

b) Buffer solution

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7
Q
  1. The relative solubility of solute in both the phases
    determines the
    a) Rate of movement of solvent
    b) Rate of disappearance of solvent
    c) Rate of movement of solute
    d) Rate of disappearance of solute
A

c) Rate of movement of solute

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8
Q
  1. If the mobile phase is gas, movement of solute is
    determined by its
    a) Boiling point
    b) Melting point
    c) Solubility
    d) Volatility
A

d) Volatility

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9
Q
  1. Column chromatography is based on the principle of
    a) Ion-exchange
    b) Exclusion principle
    c) Differential adsorption
    d) Absorption
A

c) Differential adsorption

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10
Q
  1. Arrange the following compounds in order of their
    increasing adsorption tendencies.
    a) Cellulose&raquo_space; starch&raquo_space; calcium carbonate&raquo_space; alumina
    b) Cellulose&raquo_space; starch&raquo_space; alumina&raquo_space; charcoal
    c) Charcoal&raquo_space; cellulose&raquo_space; alumina&raquo_space; starch
    d) Calcium carbonate&raquo_space;; alumina&raquo_space; starch&raquo_space; cellulose
A

a) Cellulose&raquo_space; starch&raquo_space; calcium carbonate&raquo_space; alumina

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11
Q
  1. Select the correct statement from the following options.
    a) The lesser the polarity of solute, more strongly it will be
    adsorbed on a polar surface
    b) The greater the polarity of solute, more weakly it will be
    adsorbed on a polar surface
    c) The greater the polarity of solute, more strongly it will
    be adsorbed on a polar surface
    d) All of the mentioned option
A

c) The greater the polarity of solute, more strongly it will
be adsorbed on a polar surface

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11
Q
  1. What is the factor responsible for the separation in
    column chromatography?
    a) Polarity differences between the solvent
    b) Polarity differences between the solute
    c) Polarity indifference between the solvent
    d) Polarity indifference between the solute
A

b) Polarity differences between the solute

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12
Q
  1. The correct order of increasing strength of adsorption is
    a) Alkanes&raquo_space; Esters&raquo_space; Aldehydes&raquo_space; Phenols&raquo_space; Ketones
    b) Aldehydes&raquo_space; Phenols&raquo_space; Ketones&raquo_space; Esters&raquo_space; Alkanes
    c) Aldehydes&raquo_space; Ketones&raquo_space; Esters&raquo_space; Alkanes&raquo_space; Phenols
    d) Alkanes&raquo_space; Esters&raquo_space; Ketones&raquo_space; Aldehydes&raquo_space; Phenols
A

d) Alkanes&raquo_space; Esters&raquo_space; Ketones&raquo_space; Aldehydes&raquo_space; Phenols

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13
Q
  1. The components of the mixture in column
    chromatography are eluted in order of
    a) Increasing polarity and decreasing distribution ratio
    b) Increasing polarity and increasing distribution ratio
    c) Decreasing polarity and increasing distribution ratio
    d) Decreasing polarity and decreasing distribution ratio
A

b) Increasing polarity and increasing distribution ratio

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14
Q
  1. The elution power of a solvent is determined by
    a) Its overall polarity
    b) The polarity of the stationary phase
    c) The nature of the sample components
    d) All of the mentioned
A

d) All of the mentioned

15
Q
  1. Which of the following is separated through column
    chromatography?
    a) Chlorophyll and carotenoids
    b) Inorganic cations or complexes
    c) Sugar derivatives
    d) Amino acids formed by hydrolysis of a protein molecule
A

a) Chlorophyll and carotenoids

16
Q
  1. The mixture of petroleum ether and benzene is used in
    the elution ratio of
    a) 1 : 2
    b) 1 : 5
    c) 1 : 9
    d) 1 : 12
A

c) 1 : 9

17
Q
  1. Chloroform fraction is eluted from the column by
    passing chloroform through the column which acts as
    a) Eluter
    b) Eluant
    c) Elution
    d) None of the mentioned option
A

b) Eluant

18
Q
  1. The principle on which thin layer chromatography is
    based is that the
    a) Different compounds are absorbed on an absorbent to
    different degrees
    b) Different compounds are absorbed on an absorbent to
    same degrees
    c) Different compounds are adsorbed on an adsorbent to
    different degrees
    d) Different compounds are absorbed on an absorbent to
    same degrees
A

c) Different compounds are adsorbed on an adsorbent to
different degrees

19
Q
  1. The size of a thin layer of adsorbent is about
    a) 0.1 mm
    b) 0.2 mm
    c) 0.3 mm
    d) 0.4 mm
A

b) 0.2 mm

20
Q
  1. The eluant filled in the closed jar is
    a) Mixture of gases
    b) Mixture of a liquid and a gas
    c) Mixture of solids
    d) Mixture of liquids
A

d) Mixture of liquids

21
Q
  1. Select the incorrect statement from the following
    options.
    a) The spots of colorless compounds are invisible to the
    eyes
    b) These spots can be detected by putting the plate under
    ultraviolet light
    c) These spots can be detected by placing the plate in a
    covered jar containing iodine crystals
    d) None of the mentioned
A

d) None of the mentioned

22
Q
  1. The relative adsorption of each components of the
    mixture is expressed in terms of its
    a) Acceleration factor
    b) Retardation factor
    c) Both acceleration and retardation factor
    d) None of the mentioned
A

b) Retardation factor

23
Q
  1. Select the correct statement from the following.
    a) Paper chromatography is a type of partition
    chromatography
    b) A special quality paper is used in paper chromatography
    c) Chromatography paper contains water trapped in it,
    which acts as stationary phase
    d) All of the mentioned
A

d) All of the mentioned

24
Q
  1. The chromaplate or thin layer chromatography plate is
    made up of
    a) Glass
    b) Wood
    c) Fibre
    d) Metal
A

a) Glass

25
Q
  1. Amino acids detected by spraying the plate with
    ninhydrin solution is an example of
    a) Column chromatography
    b) Thin layer chromatography
    c) Paper chromatography
    d) Liquid chromatography
A

c) Paper chromatography

26
Q
  1. Paper chromatography is based on continuous
    differential partitioning of components of a mixture
    between stationary and mobile phases.
    a) True
    b) False
A

a) True

27
Q
  1. Retardation factor is the ratio of
    a) Distance moved by substance from base line to distance
    moved by the solvent from base line
    b) Distance moved by solvent from base line to distance
    moved by the substance from base line
    c) Distance moved by substance from top line to distance
    moved by the solvent from top line
    d) Distance moved by solvent from top line to distance
    moved by the substance from top line
A

a) Distance moved by substance from base line to distance
moved by the solvent from base line

28
Q
  1. What are the uses of partition chromatography?
    a) Separation of amino acids
    b) Analysis of closely related aliphatic alcohols
    c) Separation of sugar derivatives
    d) All of the mentioned
A

d) All of the mentioned

29
Q
  1. Which type of chromatography is used for the structural
    analysis?
    a) Column chromatography
    b) Paper chromatography
    c) Partition chromatography
    d) Affinity chromatography
A

b) Paper chromatography

30
Q
  1. Which of the following is not done using column
    chromatography?
    a) The identification of unknown compounds
    b) The determination of homogeneity of chemical
    substances
    c) Separation of inorganic cations or complexes
    d) Separation of geometric isomers
A

c) Separation of inorganic cations or complexes

31
Q
  1. Affinity chromatography is used for the analysis and
    isolation of
    a) Insoluble starch substances
    b) Enzyme tyrosinase
    c) Antibodies bound with a covalently-attached antigen on
    cellulose column
    d) All of the mentioned
A

d) All of the mentioned

32
Q
  1. Purification of enzymes and proteins is done using
    a) Affinity chromatography
    b) Liquid chromatography
    c) Column chromatography
    d) Thin layer chromatography
A

a) Affinity chromatography

33
Q
  1. Which of the following is not an application of high
    performance liquid chromatography?
    a) Analysis of proteins, drugs and explosives
    b) Separation of pharmaceutical drugs
    c) Elimination of undesirable substances from blood
    d) Separation of lipids, fatty acids and steroids
A

c) Elimination of undesirable substances from blood

33
Q
  1. The checking of purity of samples is the application of
    thin layer chromatography.
    a) True
    b) False
A

a) True

34
Q
  1. The analysis and separation of industrial products like
    soap and synthetic detergents is done using
    a) Thin layer chromatography
    b) Gas chromatography
    c) Ion exchange chromatography
    d) Partition chromatography
A

b) Gas chromatography

35
Q
  1. Which of the following is the application of ion
    exchange chromatography?
    a) The softening of hard water
    b) The demineralisation of water
    c) The separation and determination of anions
    d) All of the mentioned
A

d) All of the mentioned

36
Q
  1. The quantitative analysis is done using
    a) Ion exchange chromatography
    b) Thin layer chromatography
    c) Gas chromatography
    d) Liquid chromatography
A

c) Gas chromatography

Chemical Technician (Analytical Chemistry) (16 decks)

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