Introduction to Artificial Reproductive Technologies (ART)

Question 1

When comparing standard in vitro fertilization (IVF) to ICSI, the main difference between these two techniques is:

Answer 1

The way sperm reach the ooplasma of the oocyte

Question 2

What are the two types of In Vitro Fertilization?

Answer 2

• Standard IVF (Bovine)
• Intracytoplasmic Sperm Injection (ICSI) Horse

Question 3

(T/F) When transferring an embryo into a recipient cow, care must be taken to place the embryo in the uterine body so it can migrate throughout the uterus for successful maternal recognition of pregnancy

Answer 3

False, Embryo MUST be deposited on the uterine horn ipsilateral to the CL to assure proper Maternal Recognition of Pregnancy

Question 4

Which would NOT be an advantage of Artificial Insemination over traditional breeding?

Answer 4

Higher pregnancy rates

Question 5

What are the ET Failures of the following?
Ovary:
Oviduct:
Uterus:
Cervix:

Answer 5

Ovary: Ovulation Failure
Oviduct: Obstruction
Uterus: Cysts, Chronic Infection, Fibrosis
Cervix: Laceration, Fibrosis

Question 6

When performing AI on a mare, where is the semen deposited?

Answer 6

Body of the Uterus

Question 7

Equine Oocyte Collection:
In vivo, mature oocytes are collected just prior to the expected time of OV and are arrested in _________________ stage

Answer 7

Metaphase-II