Introduction Flashcards
branch of anatomy that deals with the study of tissues
histology
scientific study of the fine details of cells and tissues using a microscope
histology
greek word of histology
histos
meaning of histos
tissue
meaning of logos
study
study of the identification and distribution of chemical compounds
histochemistry
way to understand the function of cells/tissues/organs/systems
education
to inform treatment options
diagnosis
histology is indispensable in biological research
research
to determine possible causes of sudden death
forensic investigations (autopsy)
for tissues from archeological sites
archeology
he introduced the concept of tissue in anatomy
xavier bichat
year when xavier bichat introduced the concept of tissue
1801
owns a book in year 1819
karl meyer
the science that deals with the use of microscope
microscopy
the technology of making very small things visible to human eye
microscopy
the ratio between the size of an image produced by the microscope and its actual size
magnification
a measure of clarity
resolution
the ability of visualized a particular cell structure
contrast
10x
low power
45x, 60x
high power
100x
oil immersion
the power of enlargement of image on an object
magnification
refers to the light intensity
contrast
the resolving power of a microscope
resolution
the _________ the resolving power of the microscope, the more detailed the specimen
greater
types of microscopy
light microscopy, electron microscopy
light as a source of illumination
light microscopy
basic tool of a histologist
light microscope
use visible light as the source of illumination
bright field microscopy
the background remains dark
dark-field microscopy
operates on the principle of scattering
dark-field microscopy
where an entire organism is small enough to be placed directly onto a microscope slide
whole mount
where specimens are supported in some way so that very thin slices can be cut
sections
where the specimen consists of cell suspended in fluid
smears
treatment of tissue with chemicals
fixation
removal of water
dehydration
used for dehydration
ethyl alcohol
process of making tissue transparent
clearing
complete removal of clearing agents
impregnation
the casting of tissue in melted paraffin
embedding
process of cutting tissue into sections
sectioning/cutting
process of placing tissue section on adhesive glass slide
mounting
process of coloring tissues for easy visualization
staining
preserves the chemical of nature of the tissue to be demonstrated further
histochemical fixative
preserves the anatomy of the tissue
microanatomic fixatives
preserves the intracellular structures or inclusion
cytologic fixatives
staining method used to detect polysaccharides
periodic acid schiff (PAS)
used to identify chromosomal material
feulgen reacetion
method for localizing alkaline phosphatase enzyme
gomori-takamatsu
substance used to set dyes on tissue sections by forming a coordination complex
mordant
stain with color contrasting the principal stain
counterstain
a method of staining tissue that gives information on the presence and location of macromolecules
histochemistry
uses fluoroscein-labeled antibodies
immunocytochemistry
a method that incorporates radioactive isotopes
autoradiography
this corresponds to one-millionth of a meter
micrometer
this corresponds to one-billionth of a meter
nanometer
this corresponds to one ten-billionth of a meter
angstrom