INTRO TO UA Flashcards
Urine composition
Water (95-97%)
Solids (3-5%)
TYPE OF URINE SPX
For routine and qualitative UA
Random/occasional/single
TYPE OF URINE SPX
Ideal spx for routine UA and Pregnancy Test (hCG)
1ST MORNING
— most concentrated, acidic; well preservation on cells and casts
— may be used for evaluation of orthostatic proteinuria
TYPE OF URINE SPX
For diabetic screening/monitoring
2-HOUR POSTPRANDIAL
TYPE OF URINE SPX
May be optional with blood samples in glucose tolerance Test
GLUCOSE TOLERANCE
TYPE OF URINE SPX
For routine screening and bacterial culture
MIDSTREAM CLEAN CATCH
TYPE OF URINE SPX
For bacterial culture
CATHETERIZED
TYPE OF URINE SPX
Bladder urine for anaerobic bacterial culture and urine cytology
SUPRAPUBIC ASPIRATION
TYPE OF URINE SPX
Use of soft, clear plastic bag with adhesive
PEDIATRIC SPECIMEN
TYPE OF URINE SPX
For prostatic infection determination
3 GLASS TECHNIQUE
(+) for Prostatic Infectiom
If the number of WBC and bacteria in the 3rd specimen is 10x greater than thane 1st specimen.
*** 2nd specimen = CONTROL
TYPE OF URINE SPX
Begins and ends with an empty bladder; requires preservation
24 HOUR URINE
TYPE OF URINE SPX
For Addis Count
12 HOUR URINE
TYPE OF URINE SPX
Used for nitric determination
4 HOUR
— urine remains in the bladder for at least 4 hours before being collected
TYPE OF URINE SPX
For urobilinogen determination
AFTERNOON URINE (2-4PM)
The process that provides documentation of proper sample identification from the time of collection to the receipt of laboratory results
CHAIN OF CUSTODY (COC)
Required urine volume for drug testing
30-45ml
Required urine container volume for drug testing
60ml
Temperature of urine within 4 mins for drug testing
32.5—37.7 C
This is added to the toilet water reservoir to prevent specimen adulteration
BLUEING AGENT (DYE)
Changes in unpreserved urine
INCREASED: • pH • Bacteria • Odor • Nitrite
DARKENED/MODIFIED:
• Colour
DECREASED • Clarity • Glucose • Ketone • Bilirubin • Urobilinogen • RBC/WBC/Cast • Trichomonas
URINE PRESERVATIVES
Does not interfere with chemical tests; may precipitate amorphous phosphates and urates; prevents bacteria growth for 24 hours
REFRIGERATION
URINE PRESERVATIVES
Preserves glucose and sediments well; interferes with acid precipitation test for protein
THYMOL
URINE PRESERVATIVES
Preserves protein & formed elements well; keeps pH about 6.0 Bacteriostatic at 18 g/L; can use for culture transport
BORIC ACID
URINE PRESERVATIVES
Excellent sediment preservative
FORMALDEHYDE/FORMALIN
URINE PRESERVATIVES
Does not interfere with routine tests; floats on a urine surface
TOLUENE
URINE PRESERVATIVES
Prevents glycolysis, good preservative for drug analysis; may use sodium benzoate instead of fluoride for reagent strip testing
SODIUM FLUORIDE
URINE PRESERVATIVES
Does not interfere with routine tests; causes an odor change
PHENOL
URINE PRESERVATIVES
Preserves cellular elements; used for cytology studies (50ml urine)
SACCOMANNO FIXATIVE (50% ethanol + 2% carbowax)