INTRO TO MICROPARA

Question 1

specialized area of biology that deals with organisms too small to be seen without
magnification

Answer 1

MICROBIOLOGY

Question 2

the study of parasites, their hosts, and the relationship between them

Answer 2

PARASITOLOGY

Question 3

give me the 7 microorganisms

Answer 3

BACTERIA, ARCHAEA, PROTOZOA, FUNGI, HELMINTHS, VIRUSES, ALGAE

Question 4

• Prokaryotic
   Cell wall with peptidoglycan (most)
   Unicellular
   Reproduce by binary fission (asexually)
   Circular DNA
   Some are autotrophic, some are heterotrophic

Answer 4

BACTERIA

Question 5

 Prokaryotic
 Cell wall lacks peptidoglycan
 Unicellular
 Reproduce by binary fission (asexually)
 Extremophiles
 Thermophiles , Halophiles , Methanogens

Answer 5

ARCHAEA

Question 6

WHAT ARE THE 3 EXTREMOPHILES

Answer 6

Thermophiles , Halophiles , Methanogens

Question 7

 Eukaryotic
 Lacks cell walls
 Usually heterotrophic
 Unicellular
 Can reproduce asexually and sexually
 Moves by: Pseudopods , Flagella , Cilia , Some are
non -motile

Answer 7

PROTOZOA

Question 8

PROTOZOA MOVES BY:

Answer 8

Pseudopods , Flagella , Cilia , Some are non -motile

Question 9

 Eukaryotic
 Cell wall has chitin
 Heterotrophic
 Unicellular (yeast) or multicellular (molds and
mushroom)
 Can reproduce asexually and sexually
 Linear DNA

Answer 9

FUNGI

Question 10

 Flatworms and roundworms
 No cell wall
 Heterotrophic
 Can reproduce sexually and asexually
 Have microscopic stages

Answer 10

HELMINTHS

Question 11

 Acellular
 Obligate intracellular parasite
 DNA or RNA
 May be enveloped or naked
 A capsid (protein coat) is required

Answer 11

VIRUSES

Question 12

 Eukaryotic
 Cell wall has cellulose
 Photosynthetic
 Unicellular or multicellular
 Can reproduce sexually or asexually
 Often contains pigments: green , red, or brown

Answer 12

ALGAE

Question 13

WHAT ARE THE 3 PIGMENTS THAT ALGAE CONTAINS: ____, ___, ___

Answer 13

GREEN, RED & BROWN

Question 14

 Magnification : up to about 1000×

Answer 14

LIGHT MICROSCOPY

Question 15

Uses visible or ultraviolet light to produce
an image

Answer 15

LIGHT MICROSCOPY

Question 16

 Commonly used in a wide variety of laboratory
applications as the standard method.

Answer 16

BRIGHTFIELD MICROSCOPY

Question 17

 Produces an image on a bright background.

Answer 17

BRIGHTFIELD MICROSCOPY

Question 18

GRAM (+)

Answer 18

PURPLE

Question 19

GRAM (-)

Answer 19

RED/PINKISH

Question 20

COCCI means

Answer 20

ROUND

Question 21

BACILLI means

Answer 21

ROD

Question 22

What bacteria is found in yogurt?

Answer 22

Lactobacillus acidophilus

Question 23

What bacteria is found in yakult?

Answer 23

Lactobacillus casei

Question 24

 Increases contrast without staining by producing a
bright image on a darker background.

Answer 24

DARKFIELD MICROSCOPY

Question 25

 Uses refraction and interference caused by
structures in the specimen to create high contrast,
high resolution images without staining.

Answer 25

PHASE CONTRAST MICROSCOPY

Question 26

Uses interference patterns to enhance contrast between different features of specimen to produce high-contrast images with 3D appearance.

Answer 26

DIFFERENTIAL INTERFERENCE CONTRAST (DIC) MICROSCOPY

Question 27

 Useful for viewing live specimens.

Answer 27

DARKFIELD MICROSCOPY

Question 28

 Useful for viewing live specimens, and structures
such as endospores and organelles.

Answer 28

PHASE CONTRAST MICROSCOPY

Question 29

Uses interference patterns to enhance contrast
between different features of specimen to produce
high-contrast images with 3D appearance.

Answer 29

DIFFERENTIAL INTERFERENCE CONTRAST (DIC) MICROSCOPY

Question 30

 Uses fluorescent stains to produce an image.

Answer 30

FLUORESCENCE MICROSCOPY

Question 31

 Useful for identifying pathogens, to find particular
species, to distinguish living from dead cells, or to find locations of particular molecules within a cell.

Answer 31

FLUORESCENCE MICROSCOPY

Question 32

Uses a laser to scan multiple z-planes successively, producing numerous two dimensional, high resolution images at various depths that can be constructed in a 3D image by a computer.

Answer 32

CONFOCAL MICROSCOPY

Question 33

 Useful for examining thick specimens such as biofilms.

Answer 33

CONFOCAL MICROSCOPY

Question 34

 Uses electron beams focused with magnets to
produce an image.

Answer 34

ELECTRON MICROSCOPY

Question 35

 Uses electron beams focused with magnets to
produce an image.

Answer 35

ELECTRON MICROSCOPY

Question 36

 Uses electron beams that pass through a specimen
to visualize small images.

 Useful to observe small, thin specimens such as
tissue sections and subcellular structures.

Answer 36

TRANSMISSION ELECTRON MICROSCOPY (TEM)

Question 37

 Uses electron beams to visualize surfaces.

 Useful to observe the three-dimensional surface
details of specimens

Answer 37

SCANNING ELECTRON MICROSCOPY (SEM)