Intro To Microbiology Flashcards
State the pathenogenesis of C. Dificile
- Antibiotic Treatment
- Disruption of normal colonic microflora
- Exposure to C. dificile spores, germination and colonization
- Toxin Production
- Damage to Colonic mucosa, inflammation and diarrhea
- Antibiotic Treatment
RELAPSE
What is C. Dificile
- Rod shaped, spore forming, gram positive bacterium
- Strict anaerobe
- Human pathogen –> diarrhea and pseudomenbranous colitis
- Synthesizes two large toxin proteins (TcdA and TcdB) –> disrupt intestinal epithelium
What do Eukaryotes have that Prokaryotes do not?
- Nuclear Membrane
- Membrane bound organelles
- Endocytosis
State the three types of bacterial shape
- Rod (bacilli)
- Sphere (cocci)
- Spiral (spirala)
Define endotoxin
A toxin that is present inside a bacterial cell and is released when the cell disintegrates. It is sometimes responsible for the characteristic symptoms of a disease
What structure is unique to bacteria and a target for antibiotics?
Bacteria Cell Wall
What does the bacteria cell wall consist of and what is its function?
-Consists of peptidoglycan (polysaccharide chain crosslinked by short chain AA)
- Gives barrier function
- Cell shape
What does the polysaccharide peptidoglycan chain consist of (2)?
N-acetylmuramic acid
N-acetylglucosamine
What is the antibiotic that kills newly formed cross links but not already formed cross links?
Penecillin
What is gram positive?
Where bacteria have a thick cell wall (peptidoglycan containing layer)
NO OUTER MEMBRANE
What is a gram negative?
Where bacteria have 2 cell membranes and thin peptidoglycan layer
-stain pink in slide
What are the two bacterial growth requirements?
- autotrophs
2. prototrophs
What is a prototroph?
Where bacteria with no requirements for organic compounds other than a carbon source (sugar and nitrogen)
What is an autotroph?
Bacteria that have more complex requirements, usually requiring AA and/or vitamins, purines
EX. C. dificile
What is the Lag phase in bacterial growth?
Where bacteria senses that it is in a nutrient rich environment