Bacteriophages L5 Flashcards

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1
Q

What is a Bacteriophage?

A
  • Obligate Parasite

- Virus

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2
Q

T/F Viral genome is only double stranded

A

False.

Can be double stranded or single stranded

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3
Q

State the stages of Infections (7)

A
  1. Adsorption
  2. Penetration
  3. Gene expression
  4. Nucleic acid Replication
  5. Synthesis of structural proteins
  6. Assembly of Virions
  7. Lysis-Release
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4
Q

What are the two stages of phages?

A

Early and Late

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5
Q

Is T7 Phage factor independent of factor dependent?

A

BOTH

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6
Q

What happens 12 mins after infection?

A

Early stops completely and late stage keeps going

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7
Q

Why aren’t the RNS’s made at the beginning?

A

Because the GP1 encodes for the late RNA, which is after the start of the early RNA

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8
Q

What does the early RNA stop after late starts?

A

GP2 stops the early transcription

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9
Q

What are the two phases that phages can choose?

A

Lytic or Temperate (lysogenic)

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10
Q

Bacteriophage lambda is what type of phage?

A

Temperate

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11
Q

What are the two antiterminators in the lytic process?

A

N and Q

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12
Q

What stage do you see the N antiterminator?

A

Middle Stage

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13
Q

What stage do you see Q antiterminator?

A

Late Stage

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14
Q

What gene is only gene NOT transcribed in the Lytic phage?

A

CI

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15
Q

What does CI do?

A

Its a repressor for Lytic Growth

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16
Q

What makes the decision of Lysis?

A

Rich Medium –> HIGH levels of protease

17
Q

What makes the decision of Lysogeny?

A

Poor medium –> low levels of protease

18
Q

How does lambda get out of the chromosome?

A

Induction

19
Q

How does induction happen?

A

SOS response

20
Q

What elicits the SOS response?

A

DNA damage

21
Q

Is SOS expressed all the time?

A

No

22
Q

What represses the SOS response?

A

LexA

23
Q

What happens after Induction? (3)

A
  1. Phage genes are expressed
  2. Phage development proceeds
  3. Phage genome is excised from the chromosome
24
Q

T/F Only temperate phages can do specialized transduction

A

True