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Histology > Intro to Histology > Flashcards

Intro to Histology Flashcards

1
Q

Tissue structure is closely related to tissue function?

True or false

A

True

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2
Q

What are the main tissue types?

A

Epithelial tissue
Connective tissue
Muscle tissue
Nervous tissue

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3
Q

Describe the function of epithelial tissue

A

Barrier and lining tissue

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4
Q

Give an example of epithelial tissue

A

Outer layer of skin

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5
Q

Describe the function of Connective tissue

A

Provides structural and functional support

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6
Q

Provide an example of connective tissue

A

cartilage, bone

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7
Q

Describe function of muscle tissue

A

Specialised for contraction

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8
Q

Provide and example of MUSCLE TISSUE

A

Cardiac cells

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9
Q

Describe the function of nervous tissue

A

carries information throughout the body vis electrical impulses

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10
Q

provide an example of nervous tissue

A

nerves

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11
Q

What do light microscopes show

A

reveal basic cellular structure

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12
Q

what do electron microscopes show

A

reveal ultrastructure

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13
Q

What is the most common staining technique

A

Haematoxylin & Eosin (H&E)

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14
Q

what is histology?

A

the study of tissues and cells

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15
Q

What do tissues consist of?

A

a group of cells that have similar structure and that function together as a unit.

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16
Q

Describe the key stages (7) required to collect and prepare tissues for histological examination

A
  1. Specimen collection - incision or biopsy
  2. fixation - prevents decomposition (preserves structural arrangement)
  3. Dehydration
  4. embedding
  5. sectioning
  6. staining
  7. viewing
17
Q

Describe H&E staining

A

Commonly used
Strains acidic structures (nucleic acids) blue/purple
Eosin is acidic and stains basic structures red or pink (cytoplasmic proteins)

18
Q

Periodic acid-schiff reaction (PAS)

A

Stains complex carbohydrates magenta
Mucins produced by goblet cells, brush boarders and basement membranes are all PAS-positive

19
Q

what is the Masson Trichome stain?

A
  • Stains connective tissues.
  • Nuclei are other basophilic structures stain blue/purple.
  • Collagen stains green/blue.
  • Cytoplasm, muscle, red blood cells and keratin stain red.
20
Q

What is Immunohistochemistry?

A
  • Antibodies required to identify substance of interest.
  • Utilises antibody specificity for antigen
    Antibody conjugated to an indicator (enzymatic or fluorescent).
21
Q

Name the four types of tissue?

A

Connective, Epithlieum, nervous and muscle

Histology (9 decks)

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